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Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains
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Neuroscience
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JoVE Journal Neuroscience
Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains

Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains

DOI:
10.3791/63679-v

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05:17 min

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March 31, 2022

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1Edmond and Lily Safra Center for Brain Sciences (ELSC),The Hebrew University of Jerusalem

Chapters

  • 00:04Introduction
  • 00:46Hydrogel Polymerization and Tissue Clearing
  • 02:32Chamber Preparation
  • 03:28Results: Visualization of Astrocytes and Neurons in Cleared Brains
  • 04:36Conclusion

Summary

Automatic Translation

Automatic Translation

Combining viral vector transduction and brain clearing using the CLARITY method allows the investigation of a large number of neurons and astrocytes simultaneously.

Tags

Keywords: Spatial InteractionAstrocytesNeuronsBrainTransparencyHydrogelClearing SolutionRefractive Index Matching Solution

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