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Journal / Genetics / 反向遗传学设计正义RNA病毒变异株
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Genetics
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JoVE Journal Genetics
反向遗传学设计正义RNA病毒变异株
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反向遗传学设计正义RNA病毒变异株

Article DOI: 10.3791/63685-v • 15:49 min • June 9th, 2022
June 9th, 2022 •
1, 1, 2, 1
1Virology Section, Department of Life Sciences, Shiv Nadar University, 2Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences

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该协议描述了一种通过使用易出错的PCR和反向遗传学结合全长突变RNA合成在丙型肝炎病毒基因组中引入可控遗传多样性的方法。该方法为表型选择提供了一个模型，可用于10 kb长的正义RNA病毒基因组。

Tags

反向遗传学， 正义RNA病毒， 变体， 定向进化， RNA基因组， 容易出错的PCR， 病毒突变体， 感兴趣的表型， 全长突变体RNA合成（FL-MRS）， 文库， 遗传多样性， DNA聚合酶保真度， 模板， 突变RNA合成， 宿主细胞， 非选择输入池， 病毒定向进化， 丙型肝炎病毒（HCV）， JFH1分离株， 适应， 复制， 病毒基因， 发病机制， 抗病毒耐药性
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