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Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
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Neuroscience
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JoVE Journal Neuroscience
Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

DOI:
10.3791/63816-v

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09:00 min

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April 28, 2022

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, ,
1Department of Neurology,Northwestern University Feinberg School of Medicine

Chapters

  • 00:04Introduction
  • 01:01Enrichment of SDS Insoluble Material
  • 03:29Amyloid Purification
  • 04:17Methanol Chloroform Precipitation
  • 04:58Trypsin Digestion
  • 06:02Peptide Cleanup
  • 07:12Results: Confirmation and Validation of Amyloid Purification
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

This biochemical purification method with mass spectrometry-based proteomic analysis facilitates the robust characterization of amyloid fibril cores, which may accelerate the identification of targets for preventing Alzheimer's disease.

Tags

Keywords: Amyloid FibrilsAmyloid PlaquesAlzheimer’s DiseaseProtein PurificationProteomic CharacterizationHomogenizationSucrose GradientProtein AggregatesProtein Deposits

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