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Molecular and Immunologic Techniques in a Genetically Engineered Mouse Model of Gastrointestinal Stromal Tumor
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Cancer Research
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JoVE Journal Cancer Research
Molecular and Immunologic Techniques in a Genetically Engineered Mouse Model of Gastrointestinal Stromal Tumor

Molecular and Immunologic Techniques in a Genetically Engineered Mouse Model of Gastrointestinal Stromal Tumor

DOI:
10.3791/63853-v

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07:21 min

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May 02, 2022

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, , , , ,
1Department of Surgery,Hospital of the University of Pennsylvania

Chapters

  • 00:05Introduction
  • 00:42KitV558Δ/+ Mouse Organ Harvest
  • 02:26Single-Cell Suspension of Mesenteric Lymph Node
  • 03:09Single-Cell Suspension of GIST
  • 04:22Single-Cell Suspension of Cecum
  • 06:06Results: Investigation of Therapeutics in an Immunocompetent Mouse Model
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this manuscript is to describe the KitV558Δ/+ mouse model and techniques for successful dissection and processing of mouse specimens.

Tags

Keywords: Genetically Engineered Mouse ModelGastrointestinal Stromal Tumor (GIST)ImmunocompetentMolecular TechniquesImmunologic TechniquesTyrosine Kinase InhibitorsImmunotherapiesCecumMesenteric Lymph NodeSingle-cell SuspensionFlow Cytometry

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