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JoVE Journal Developmental Biology
Isolering af preadipocytter fra broilerkyllingembryoner
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Isolering af preadipocytter fra broilerkyllingembryoner

Article DOI: 10.3791/63861-v • 06:32 min • August 4th, 2022
August 4th, 2022 •
1, 1, 1, 1, 1
1Department of Animal Science, University of Tennessee

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Denne protokol beskriver en simpel metode til isolering af præadipocytter fra fedtvæv i slagtekyllinger. Denne metode muliggør isolering med højt udbytte, primær kultur og adipogen differentiering af præadipocytter. Olie Rød O farvning og lipid / DNA-plet målte den adipogene evne af isolerede celler induceret med differentieringsmedier.

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Udviklingsbiologi udgave 186
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