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Journal / Bioengineering / Developing Drosophila melanogaster Models for…
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Bioengineering

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JoVE Journal Bioengineering
Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function
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Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function

Article DOI: 10.3791/63939-v • 08:43 min • August 25th, 2022
August 25th, 2022 •
1, 1, 1, 1, 1,2, 1
1Department of Biomedical Engineering, Washington University in St. Louis, 2Department of Computer Science and Engineering, Washington University in St. Louis

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Summary
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The present protocol describes the generation of Drosophila melanogaster expressing eNpHR2.0 or ReaChR opsins in the heart for OCT imaging and optogenetic heart pacing. Detailed instructions for Drosophila OCT imaging and heart beating modulation, including the simulation of restorable heart arrest, bradycardia, and tachycardia in live animals at different developmental stages, are reported.

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Drosophila Melanogaster Models Imaging Optogenetic Control Cardiac Function Human Heart Diseases Intact Animals Non-invasive OCT Imaging Optogenetic Pacing Arrhythmic Disorders Model Systems Heart Organoids Zebrafish Embryonic Throat And Heart GAL4 UAS Ops And Stocks Hand GAL4 Over TM6 Double-tubby Virgin Female Flies UAS Ops And Stocks Male Flies Semi-defined Food Bloomington Drosophila Stock Center Sucrose All-trans Retinal Ethanol Solution
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