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Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

JoVE Journal
Biology

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JoVE Journal Biology

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

DOI:

10.3791/1596-v

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09:37 min

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November 16, 2009

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Ann S. Cooper, Kylah E. Rymond, Matthew A. Ward, Easter L. Bocook, Robin L. Cooper

¹Department of Biology,University of Kentucky, Lexington

Chapters

00:00Title
00:29Introduction
00:55Unrestrained Intact Larvae: The “Ant Farm” Method
02:01Restrained Intact Larvae: The “Glue Method”
03:52Larvae Dissection
05:43Electrical Pacing
07:21Field Potentials and Intracellular Recordings
08:58Conclusion

Summary

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We present various ways to monitor heart function in the larva of Drosophila for assessing questions dealing with the function of gap junctions, ion channel mutations, modulation of pacemaker activity and pharmacological studies.

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

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