A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:21Dissection of the Drosophila Retina
- 02:39Dissection of the ommatidia
- 03:26Staining and Mounting of Detached Ommatidia
- 03:59Imaging Auto-Lysosomes in a Single Ommatidium
- 04:43Conclusion
The limiting factor in the use of the adult Drosophila eye to study neurodegeneration and cell biology is the difficult imaging of intracellular processes. We describe the dissection of single ommatidia to generate a bona-fide primary neuronal cell culture, which can be subject to drug treatment and advanced imaging.
Tags
Drosophila MelanogasterFruit FlyNeuroscience ResearchNeurodegenerative DiseasesGeneticsFly EyeNeurodegeneration ResearchAutofluorescenceIntracellular EventsAutophagy DynamicsSingle Ommatidia DissectionDentatorubro-Pallidoluysian Atrophy (DRPLA)Technique DescriptionElectrophysiological StudiesFly Models For NeurodegenerationSubcellular Events ImagingDrug AdministrationPhotoreceptor CellsMosaic Techniques
