Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma

Kel  Vin Tan; Xinxiang Yang; Chung Ying Chan; Jingjing Shi; Hing-Chiu Chang; Keith Wan-Hang Chiu; Kwan Man

doi:10.3791/63958

Journal

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Cancer Research

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Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma

JoVE Journal
Cancer Research

This content is Free Access.

JoVE Journal Cancer Research

Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma

2,184 Views

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07:47 min

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August 31, 2022

DOI:

10.3791/63958-v

DOI:

10.3791/63958-v

•

07:47 min

August 31, 2022

•

2177 Views

Kel Vin Tan*^1,2, Xinxiang Yang*³, Chung Ying Chan², Jingjing Shi¹, Hing-Chiu Chang⁴, Keith Wan-Hang Chiu^1,5, Kwan Man³

¹Department of Diagnostic Radiology, School of Clinical Medicine, LKS Faculty of Medicine,The University of Hong Kong ²Department of Oncology, MRC Oxford Institute for Radiation Oncology,University of Oxford ³Department of Surgery, School of Clinical Medicine, HKU-SZH & LKS Faculty of Medicine,The University of Hong Kong ⁴Department of Biomedical Engineering,The Chinese University of Hong Kong ⁵Department of Diagnostic and Interventional Radiology,Kwong Wah Hospital

Summary

Here, we present a protocol to create orthotopic hepatocellular carcinoma xenografts with and without hepatic artery ligation and perform non-invasive positron emission tomography (PET) imaging of tumor hypoxia using [¹⁸F]Fluoromisonidazole ([¹⁸F]FMISO) and [¹⁸F]Fluorodeoxyglucose ([¹⁸F]FDG).