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Immunology and Infection
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Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

DOI:
10.3791/64032-v

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08:53 min

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May 25, 2022

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, , , , ,
1Division of Biology and Biomedical Sciences,Washington University School of Medicine, 2Department of Obstetrics, Gynecology, and Reproductive Sciences,University of California, 3Glycobiology Research and Training Center,UCSD, 4Department of Pediatrics,Washington University School of Medicine

Chapters

  • 00:05Introduction
  • 00:52Preparation of Inoculum
  • 02:08Vaginal Pre-Lavage and Inoculation with Gardnerella
  • 03:54Dissection and Tissue Homogenization
  • 07:26Results: Enumeration of Bacteria Colonizing Mouse Vagina
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presents a mouse model of vaginal colonization with anaerobically cultured human vaginal bacteria. We focus on Gardnerella vaginalis, while including suggestions for Prevotella bivia and Fusobacterium nucleatum. This protocol can also be used as a guide for vaginal inoculations and viable recovery of other anaerobically grown bacteria.

Tags

Keywords: Anaerobic BacteriaVaginal ColonizationMurine ModelInoculationVaginal LavageColony-forming UnitsObligate AnaerobesFastidious BacteriaSpectrophotometryAnaerobic Conditions

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