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Journal / Biochemistry / Kryo-EM-datainnsamling med enkeltpartikler med…
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Biochemistry

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JoVE Journal Biochemistry
Kryo-EM-datainnsamling med enkeltpartikler med scenetilt ved bruk av Leginon
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Kryo-EM-datainnsamling med enkeltpartikler med scenetilt ved bruk av Leginon

Article DOI: 10.3791/64136-v • 04:52 min • July 1st, 2022
July 1st, 2022 •
1, 1, 2, 2,3, 1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Denne protokollen beskriver et generalisert og lett implementert skjema for skråstilt enkeltpartikkeldatainnsamling i kryo-EM-eksperimenter. En slik prosedyre er spesielt nyttig for å skaffe et EM-kart av høy kvalitet for prøver som lider av fortrinnsorienteringsskjevhet på grunn av overholdelse av luft-vanngrensesnittet.

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