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Cancer Research

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Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures
 

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Article DOI: 10.3791/64146-v
July 27th, 2022

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Summary July 27th, 2022

The present protocol describes the setting up of 3D 'on top' cultures of a non-transformed breast epithelial cell line, MCF10A, that has been modified to study Platelet Activating Factor (PAF) induced transformation. Immuno-fluorescence has been used to assess the transformation and is discussed in detail.

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