Epithelial Cell Transformation
Epithelial Cell Transformation - Related Videos
09:44
In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
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2020
This protocol provides experimental in vitro tools to evaluate the transformation of human mammary cells. Detailed steps to follow-up cell proliferation rate, anchorage-independent growth capacity, and distribution of cell lineages in 3D cultures with basement membrane matrix are described.
05:34
Oral Delivery of Transformed Yeast Cells into the Mouse Intestinal Immune System
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2025
This video showcases the oral administration of transformed yeast cells into a mouse model. The transformed yeast cells are orally administered using a gavage technique, reaching the mouse's stomach. Later, the cells migrate to the small intestine, where microfold cells engulf them and facilitate their transfer to Peyer's patches and gain access to the intestinal immune system.
11:52
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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Cited by 1 •
2017
We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.
04:33
Bacterial Transformation - Concepts
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2019
Background In early 20th century, pneumonia was accountable for a large portion of infectious disease deaths1. In order to develop an effective vaccine against pneumonia, Frederick Griffith set out to study two different strains of the Streptococcus pneumoniae: a non-virulent strain with a rough appearance (R-strain) and a virulent strain with a smooth appearance (S-strain) due to an outer polysaccharide capsule2. This outer layer of the S-strain bacteria enabled them to withstand the host...
03:24
Isolation and Culture of Murine Tongue Epithelial Cells: A Procedure to Isolate and Culture Tongue Epithelial Cells from Mouse Model
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2023
In this video, we describe the isolation of epithelial cells from a murine tongue. These cells can be further cultured to give rise to cell colonies.
FAQs
Transformation can disturb several coordinated controls at once, including regulated proliferation, cell polarity, adhesion, and responses to tissue signals. Genetic changes alter cellular instructions, whereas epigenetic changes modify gene activity without necessarily changing the underlying DNA sequence. Examining these control systems helps explain how epithelial cells acquire abnormal growth behavior during tumor development.
Epithelial–mesenchymal transition provides a framework for understanding how some transformed cells change from an organized epithelial state toward greater motility and invasive potential. This process adds a progression and spread perspective to studies focused on abnormal growth. It is therefore relevant when researchers examine how epithelial cancers may move beyond their original tissue context.
No single observation captures every feature associated with transformation. Morphology can reveal loss of normal organization, proliferation measures growth behavior, and migration assays address movement. Anchorage-independent growth and tumor formation provide additional evidence of abnormal properties. Combining these readouts allows researchers to distinguish changes in appearance from broader alterations linked to tumor development.
A typical assessment compares transformed or experimentally treated epithelial cells with appropriate nontransformed counterparts across several outcomes. Researchers examine cell morphology, proliferation, migration, anchorage-independent growth, and, when included in the study, tumor formation. This multiparameter approach connects visible cellular changes with functional behaviors relevant to abnormal growth and cancer progression.
Researchers study this process to identify oncogenic mechanisms and to clarify how epithelial cancers initiate, progress, and spread. The same analyses can evaluate whether a carcinogenic influence produces transformation-associated changes or whether a therapeutic intervention affects them. Thus, the approach supports both mechanistic cancer biology and assessment of carcinogenic or therapeutic effects.
Transformation assays can show whether epithelial cells have acquired distinct cancer-associated behaviors, such as altered morphology, increased proliferation, migration, anchorage-independent growth, or the capacity to form tumors. Interpreted together, these outcomes help relate cellular changes to stages of tumor development and provide evidence about mechanisms that may contribute to cancer progression and spread.