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En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

DOI:
10.3791/64207-v

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08:57 min

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July 27, 2022

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, ,
1Intercellular Signalling in Cardiovascular Development and Disease Laboratory,Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Ciber de Enfermedades Cardiovasculares

Chapters

  • 00:04Introduction
  • 00:50Obtention of AVC and/or OFTs
  • 02:31Whole Mount Immunofluorescence of the Mouse Embryonic Heart
  • 04:35Mounting the Mouse Embryonic AVC or OFTs
  • 06:49Results: Analysis of Valve Development in Mouse Embryonic Heart
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

Tags

En FaceEndocardial CushionPlanar MorphogenesisMouse EmbryosPrimitive EndocardiumValve DevelopmentPlanar Cell PolarityCardio DevelopmentGenotypingFixationImmunostaining

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