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En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
DOI:

08:57 min

July 27, 2022

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Chapters

  • 00:04Introduction
  • 00:50Obtention of AVC and/or OFTs
  • 02:31Whole Mount Immunofluorescence of the Mouse Embryonic Heart
  • 04:35Mounting the Mouse Embryonic AVC or OFTs
  • 06:49Results: Analysis of Valve Development in Mouse Embryonic Heart
  • 08:19Conclusion

Summary

Automatic Translation

Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

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