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CRISPR-Cas9介导的斑马鱼心脏中的精确敲入编辑
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Biology
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JoVE Journal
Biology
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
Please note that all translations are automatically generated.
Click here for the English version.
CRISPR-Cas9介导的斑马鱼心脏中的精确敲入编辑
DOI:
10.3791/64209-v
•
06:52 min
•
September 13, 2022
•
Kyle E. Simpson
,
Shoaib Faizi
,
Ravichandra Venkateshappa
,
Mandy Yip
,
Raj Johal
,
Damon Poburko
,
Yen May Cheng
,
Diana Hunter
,
Eric Lin
,
Glen F. Tibbits
,
Thomas W. Claydon
1
Department of Biomedical Physiology and Kinesiology
,
Simon Fraser University
Chapters
00:05
Introduction
00:42
Design of CRISPR Components for Precise Edits
02:56
Reporter Gene Screening of CRISPR-Cas9-Edited Larval Zebrafish
03:42
Genotyping of CRISPR-Cas9-Edited Larval Zebrafish
05:20
Results: Validation of Gene-Edits and Analysis of Cardiac Consequences
06:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种使用CRISPR-Cas9技术促进斑马鱼胚胎精确敲入编辑的方法。提出了一个表型管道,以证明这些技术对长QT综合征相关基因变异进行建模的适用性。
Tags
CRISPR-Cas9
Knock-in
Zebrafish
Long QT Syndrome
SgRNA Design
HDR Template
Sanger Sequencing
Phenotyping
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