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In vivo Imagem de cálcio de campo amplo e de dois fótons de um rato usando uma grande janela craniana
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1Department of Neurophysiology, Faculty of Medicine,University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine,University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine,The University of Tokyo
Chapters
- 00:04Introduction
- 00:51Cranial Window Construction
- 03:32Making AAV Film on Plastic Wrap Using Fibroin Solution
- 04:17Results: Evaluation of Cranial Window Made Using the PVDC Wrap Methods
- 05:56Conclusion
O presente protocolo descreve a confecção de uma grande janela craniana (6 x 3 mm2) usando envoltório de alimentos, silicone transparente e vidro de cobertura. Esta janela craniana permite experiências de imagem de cálcio in vivo de campo largo e de dois fótons no mesmo rato.
