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Preclinical Drug Testing in Scalable 3D Engineered Muscle Tissues
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Preclinical Drug Testing in Scalable 3D Engineered Muscle Tissues

Preclinical Drug Testing in Scalable 3D Engineered Muscle Tissues

DOI:
10.3791/64399-v

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08:07 min

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April 07, 2023

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, , , , , , , , ,
1Curi Bio Inc.

Chapters

  • 00:04Introduction
  • 00:56Casting Plate and Cell Preparation
  • 04:02Tissue Casting
  • 05:42Results: 3D Engineered Muscle Tissue Formation and Function Over Time
  • 07:39Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol provides methods for generating 3D engineered cardiac and skeletal muscle tissues and describes their use in preclinical drug screening modalities. The described methods utilize a magnetic sensing system to facilitate the simultaneous assessment of 24 tissues in parallel.

Tags

Magnetic SensingContractilityHigh-throughput Drug TestingIn Vitro Disease ModelingGene TherapyNeuromuscular Junction ModelThrombinRPMI MediumB27Aminocaproic AcidROCK InhibitorF10 MediumDissociation ReagentCell Suspension

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