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JoVE Journal Cancer Research
Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
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Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Article DOI: 10.3791/64484-v • 10:13 min • December 2nd, 2022
December 2nd, 2022 •
1,2, 3, 1,2, 2,3,4, 1,3, 3
1Department of Molecular Biology and Genetics, Aarhus University, 2Department of Clinical Medicine, Aarhus University, 3VPCIR Biosciences ApS, 4Department of Pathology, Aarhus University Hospital

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Summary
Automatic Translation

A protocol for the sensitive and quantitative detection of topoisomerase 1 activity using the rolling circle enhanced enzyme activity detection assay is described. The method allows detection of topoisomerase 1 activity from purified components or cell/tissue extracts. This protocol has wide-ranging applications in any field involving detection of enzymatic activity.

Tags

Rolling Circle Amplification Topoisomerase 1 Activity Crude Biological Samples Fast Detection Method Simple Protocol Gel-based Assays Sensitivity Drug Treatment Response Small Molecule Compound Screening Anti-cancer Effect Anti-infectious Diseases Effect Silicone Isolator Grid Functionalized Slide Well Maker Primer Mix Amino Primer Hybridization Chamber Closed Circular Substrates Topoisomerase 1 Enzyme Cell Extract Reaction Buffer
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