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IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
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Bioengineering
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JoVE Journal Bioengineering
IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

DOI:
10.3791/64507-v

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10:48 min

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November 13, 2023

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, , , ,
1Department of Orthopedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital,Shanghai Jiao Tong University School of Medicine

Chapters

  • 00:05Introduction
  • 00:33Preparation of the Cell-Matrix Mixture
  • 02:07Plating the Cell-Gel Mixture
  • 02:58Identifying the Cell Viability and Cellular Dendrites Using a Confocal Microscope
  • 06:00Identifying Appearance Using a Stereomicroscope
  • 06:55Identifying Mineral Deposition by XRF Assay
  • 07:45Identifying Functional Gene Expression
  • 08:25Results: Dendrite Network, Mineral Deposition, and Gene Expression of Osteogenic Cultured IDG-SW3 Cells
  • 10:04Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for culturing IDG-SW3 cells in a three-dimensional (3D) extracellular matrix.

Tags

Keywords: 3D Cell CultureExtracellular MatrixCollagen IIDG-SW3 CellsOsteogenesisGene ExpressionCell DifferentiationCell ImagingCell StainingCalcium AMEthidium Homodimer-1

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