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Neuroscience

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JoVE Journal Neuroscience
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Article DOI: 10.3791/64574-v • 12:14 min • September 14th, 2022
September 14th, 2022 •
1,2,3, 1,2, 1,2
1Department of Neurology, Yale University, 2Department of Neuroscience, Yale University, 3Interdepartmental Neuroscience Program, Yale University

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Summary
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This protocol presents a robust, detailed method to obtain highly pure synaptosomes, synaptic vesicles, and other synaptic fractions from the mouse brain. This method enables the evaluation of synaptic processes, including the biochemical analysis of protein localization and function with compartmental resolution.

Tags

Subcellular Fractionation Synaptic Components Murine Brain Synapses Molecular Processes Brain Disorders Synaptosome Structures Specific Proteins Subsynaptic Resolution Experimental Procedure Length Of Procedure Tissue Collection Buffers Bench Top Materials Collection Tubes
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