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Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

DOI:
10.3791/64586-v

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08:20 min

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May 12, 2023

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, ,
1Department of Applied Stem Cell Technologies, TechMed Centre Enschede,University of Twente, 2Centro de Biotecnología-FEMSA,Tecnologico de Monterrey, 3Department of Genetics,Harvard Medical School

Chapters

  • 00:04Introduction
  • 00:55Microfilament Preparation
  • 01:56Embryoid Body Formation
  • 03:42Neuroepithelial Induction
  • 04:21Organoid Embedding and Differentiation
  • 06:39Results: Characterization of the Organoids
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol provides a step-by-step procedure for the reproducible generation, maintenance, and aging of cerebral organoids derived from human-induced pluripotent stem cells (iPSCs). This method enables culturing and maturing cerebral organoids for extended periods, which facilitates the modeling of processes involved in brain aging and age-related pathogenesis.

Tags

Keywords: IPSCBrain OrganoidsAging ResearchLong-term CulturePLGA MicrofilamentsInduced Pluripotent Stem CellsCell DissociationE8 MediaCentrifugation

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