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Journal / Immunology and Infection / Generering av genetiskt modifierade Plasmodium…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generering av genetiskt modifierade Plasmodium berghei Sporozoites
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Journal (Immunology and Infection)
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Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)
Journal (Immunology and Infection)
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Generering av genetiskt modifierade Plasmodium berghei Sporozoites

Article DOI: 10.3791/64992-v • 10:16 min • May 5th, 2023
May 5th, 2023 •
1, 1,2
1Center for Tropical and Emerging Global Diseases, University of Georgia, 2Department of Cellular Biology, University of Georgia

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Malaria överförs genom inokulering av sporozoitstadiet av Plasmodium av infekterade myggor. Transgent Plasmodium har gjort det möjligt för oss att förstå malarians biologi bättre och har bidragit direkt till utvecklingen av malariavaccin. Här beskriver vi en strömlinjeformad metodik för att generera transgena Plasmodium berghei-sporozoiter.

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Genetiskt modifierad Plasmodium berghei Sporozoites malaria parasitplasmodium anophelesmyggor leverutvecklingsmyggor klinisk malaria immunsvar transgena parasiter blodstadium P. berghei myggans spottkörtlar in vivo-experiment in vitro-experiment grönt fluorescerande protein (GFP) levermalaria
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