What are endodermal hepatic organoids?

Endodermal hepatic organoids (eHEPOs) are 3D liver models derived from endodermal stem cells that mimic liver tissue functions.

How long does it take to produce eHEPOs?

It takes approximately 2 weeks to produce endodermal hepatic organoids using the described protocol.

Can eHEPOs expand over time?

Yes, eHEPOs can expand long-term, exceeding 1 year, without losing their differentiation and functionality.

What is the significance of this research?

This research addresses the need for reproducible and functional organoid models for liver studies, which is essential for understanding liver diseases.

Are there any technological requirements for this protocol?

Yes, specific cell culture and assessment technologies are required to generate and maintain eHEPOs effectively.

What applications can eHEPOs be used for?

eHEPOs can be used for drug testing, disease modeling, and understanding liver physiology.

Is there a comparison with other organoid systems?

The protocol may provide a more reliable approach compared to other liver organoid systems in terms of reproducibility and functionality.

Generation of Functional Endodermal Hepatic Organoids

1.3K views

Cited by 1

08:45 min

May 2nd, 2025

10.3791/65027-v

May 2nd, 2025

1.3K views

Soheil Akbari*¹ , Nevin Ersoy*¹^,²^,³ , Alper Bagriyanik¹^,²^,⁴ , Nur Arslan¹^,⁵ , Tamer Tevfik Önder⁶ , Esra Erdal¹^,⁷

¹Izmir Biomedicine and Genome Center, ²Department of Histology and Embryology, Health Science Institute, Dokuz Eylul University, ³Department of Histology and Embryology, Faculty of Medicine, Izmir Tinaztepe University, ⁴Department of Histology and Embryology, Faculty of Medicine, Dokuz Eylul University, ⁵Department of Pediatric Gastroenterology and Metabolism, Faculty of Medicine, Dokuz Eylul University, ⁶Department of Medical Biology, Faculty of Medicine, Koc University, ⁷Department of Medical Biology, Faculty of Medicine, Dokuz Eylul University

This protocol describes the generation of fast and reproducible endodermal hepatic organoids (eHEPOs). With this protocol, eHEPOs can be produced within 2 weeks and expand long-term (more than 1 year) without losing their differentiation and functionality.