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Dual-Dye optisk kortlægning af hjerter fra RyR2R2474S Knock-In mus af katekolaminerg polymorf ventrikulær takykardi
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Dual-Dye optisk kortlægning af hjerter fra RyR2R2474S Knock-In mus af katekolaminerg polymorf ventrikulær takykardi

Article DOI: 10.3791/65082-v • 09:36 min • December 22nd, 2023
December 22nd, 2023 •
*1,2, *1, 1, 1, 1, 1, 3, 1, 1,2, 1,4, 1
1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, 2Department of Cardiology, the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 4Department of Pharmacology, University of Oxford
* These authors contributed equally

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Denne protokol introducerer dobbeltfarvet optisk kortlægning af musehjerter opnået fra vildtype- og knock-in-dyr, der er ramt af katekolaminer polymorf ventrikulær takykardi, herunder elektrofysiologiske målinger af transmembranspænding og intracellulære Ca2+ transienter med høj tidsmæssig og rumlig opløsning.

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Denne måned i JoVE nummer 202
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