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Bioengineering

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JoVE Journal Bioengineering
Differensiering av humane induserte pluripotente stamceller til hjernens mikrovaskulære endotelcellelignende celler med en moden immunfenotype
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Differensiering av humane induserte pluripotente stamceller til hjernens mikrovaskulære endotelcellelignende celler med en moden immunfenotype

Article DOI: 10.3791/65134-v • 09:03 min • May 19th, 2023
May 19th, 2023 •
1, 2, 3
1Graduate School of Medicine, Yamaguchi University, 2Theodor Kocher Institute, University of Bern, 3Department of Neurotherapeutics, Yamaguchi University of Medicine

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Her beskriver vi en protokoll, den utvidede endotelcellekulturmetoden (EECM), som tillater differensiering av pluripotente stamceller til hjernens mikrovaskulære endotelcelle (BMEC)-lignende celler. Disse cellene viser endotelcelleadhesjonsmolekyluttrykk og er dermed en human blod-hjernebarrieremodell egnet til å studere immuncelleinteraksjoner in vitro.

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