Endothelial Progenitor Cells
Endothelial Progenitor Cells - Related Videos
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Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
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Cited by 16 •
2017
The goal of this protocol is to isolate endothelial progenitor cells from umbilical cord blood. Some of the applications include using these cells as a biomarker for identifying patients with cardiovascular risk, treating ischemic diseases, and creating tissue-engineered vascular and heart valve constructs.
12:17
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
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Cited by 38 •
2009
Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.
16:04
Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
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Cited by 66 •
2009
This protocol describes the isolation and subsequent expansion of mesenchymal stromal cells and endothelial colony forming cells without the use of animal serum to generate autologous pairs for experimental transplantation purposes.
11:49
Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model
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Cited by 12 •
2011
A method for seeding titanium blood-contacting biomaterials with autologous cells and testing biocompatibility is described. This method uses endothelial progenitor cells and titanium tubes, seeded within minutes of surgical implantation into porcine venae cavae. This technique is adaptable to many other implantable biomedical devices.
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Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
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Cited by 9 •
2017
Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
FAQs
Injury and low oxygen serve as signals associated with EPC mobilization and movement toward damaged regions. After reaching these sites, the cells can support endothelialization through two linked activities: incorporation into developing vascular structures and secretion of proangiogenic signals. This sequence connects local tissue damage with cellular responses that may help restore vascular function.
These mechanisms provide complementary forms of vascular support. Incorporation allows EPCs to contribute directly to endothelialization, while secreted proangiogenic signals can encourage new blood vessel formation in the surrounding tissue. Considering both activities is important when evaluating EPC performance, because vascular repair may depend on cellular participation as well as communication with nearby cells and tissues.
EPCs can originate from circulating blood or tissue, yet their identification and functional properties remain active areas of research. These differences matter because cells described as EPCs may not behave identically in vascular repair or bioengineered settings. Characterizing their source and behavior therefore helps researchers interpret regenerative outcomes and select cells for appropriate applications.
Researchers apply EPCs to vascular constructs and graft-related strategies to support endothelialization, promote blood flow, and reduce thrombosis. Their use is especially relevant where engineered materials must interact successfully with blood and surrounding tissue. Evaluating whether EPCs improve the vascular surface and associated function helps guide the development of tissue-engineered blood vessels and vascular grafts.
EPC-based approaches are investigated for tissue-engineered blood vessels, vascular grafts, implants, and cell-based therapies. Across these applications, the desired benefits include improved vascularization, support for blood flow, and lower thrombosis. The specific research goal may differ between a living tissue construct and an implant, but each application depends on improving vascular integration or repair.
Monitoring EPC behavior helps bioengineers examine how vascular repair occurs and how cells respond to damaged or oxygen-limited environments. This work can clarify vascular regeneration while also revealing why cell source and function influence outcomes. Such knowledge supports the design and assessment of grafts, implants, engineered vessels, and other regenerative strategies intended to improve vascular performance.