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Journal / Bioengineering / Differentiering av humant inducerade pluripotenta …
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Differentiering av humant inducerade pluripotenta stamceller till mikrovaskulära endotelcellliknande celler i hjärnan med en mogen immunfenotyp
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Differentiering av humant inducerade pluripotenta stamceller till mikrovaskulära endotelcellliknande celler i hjärnan med en mogen immunfenotyp

Article DOI: 10.3791/65134-v • 09:03 min • May 19th, 2023
May 19th, 2023 •
1, 2, 3
1Graduate School of Medicine, Yamaguchi University, 2Theodor Kocher Institute, University of Bern, 3Department of Neurotherapeutics, Yamaguchi University of Medicine

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Här beskrivs ett protokoll, extended endothelial cell culture method (EECM), som möjliggör differentiering av pluripotenta stamceller till hjärnans mikrovaskulära endotelcellsliknande (BMEC)-liknande celler. Dessa celler visar uttryck av endotelcelladhesionsmolekyler och är därför en human blod-hjärnbarriärmodell lämplig för att studera immuncellsinteraktioner in vitro.

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Denna månad i JoVE nummer 195
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