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07:55 min
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June 02, 2023
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Summary
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Zinc transport has proven challenging to measure due to the weak causal links to protein function and the low temporal resolution. This protocol describes a method for monitoring, with high temporal resolution, Zn2+ extrusion from living cells by utilizing a Zn2+ sensitive fluorescent dye, thus providing a direct measure of Zn2+ efflux.
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Cite this Article
Ben Yosef, T. E., Zarivach, R., Moran, A. Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay. J. Vis. Exp. (196), e65217, doi:10.3791/65217 (2023).
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