Primordial Germ Cell Cryopreservation and Revival of <em>Drosophila</em> Strains

Kaori Nishimura; Miho Asaoka; Yurina Sakamaki; Tatsuya Fukumoto; Daisuke Tanaka; Satoru Kobayashi; Toshiyuki Takano-Shimizu-Kouno

doi:10.3791/65985

Journal

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Genetics

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Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains

Primordial Germ Cell Cryopreservation and Revival of <em>Drosophila</em> Strains

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains

Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains

2,257 Views

•

06:22 min

•

December 01, 2023

DOI:

10.3791/65985-v

DOI:

10.3791/65985-v

•

06:22 min

December 01, 2023

•

19 Views

Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center,Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ³Research Center of Genetic Resources,National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

Transcript

Drosophila strain must be maintained by the frequent transfer of adult flies to new vials. This creates a danger of mutational deterioration and genetic changes. We aim to develop an alternative method for the long-term preservation of Drosophila strains without such changes.

Compare with in vivo editing, genome editing of primordial germ cells, or PGCs, could increase knock-in inefficiency and make more complicated genomic changes. Thus, a wide application of PGC manipulation facilitates genetic engineering in many fields. We now focus on xenotransplantation.

By using Drosophila melanogaster as a surrogate host, we have succeeded in reviving stock of cell species in the melanogaster species subgroup. By addressing xenotransplantation barriers and finding possible solutions, we would like to explore the potential application of PGC transplantation and cryopreservation.

Summary

A long-term preservation method for Drosophila strains as an alternative to the frequent transfer of adult flies to fresh food vials is highly desirable. This protocol describes the cryopreservation of Drosophila primordial germ cells and strain revival via their transplantation to agametic host embryos.