Generating Neural Retina from Human Pluripotent Stem Cells

Our group focuses on understanding the pathogenesis and the treatment of human retinal diseases. Our protocol describes an optimized induction technique to generate a neural retina that significantly reduce the probability of escalation and fusion to increase the success rate of retina production until death 60. The modified method is simpler and more efficient in generating neural retina, which can prove to be a better disease model for drug screening and cell therapy.

In the future, we aim to study the effects of physical chemical factors on retinal development and underlying mechanisms using our retinal organoid model. To begin, add one milliliter of 1%extracellular matrix to each well of a 6-well plate. Incubate the plate at 37 degrees Celsius in a 5%carbon dioxide atmosphere.