Modified Mouse Model of Repetitive Mild Traumatic Brain Injury Incorporating Thinned-Skull Window and Fluid Percussion

This protocol presents a modified mouse model of repetitive mild traumatic brain injury (rmTBI) induced via a closed-head injury (CHI) method. The approach features a thinned-skull window and fluid percussion to reduce the inflammation commonly caused by meninges exposure, along with improved reproducibility and accuracy in modeling rmTBI in rodents.

Traumatic brain injury is a significant health issue for the worldwide population. Our main focus is on the mechanism of TBI and the rehabilitation, with a particular focus on the mild TBI and the neuroprotective effects of access. No single animal models can adequately mimic all aspects of the human TBI due to the complexity of a clinical TBI.

Each model has its own advantages and the disadvantages when implemented for TBI research. These challenges necessitate the optimization of animal models. The closed-head injury is a common message for making the rodent models of a repeated mTBI.

However, this message is susceptible to many factors, including the impact methods used and the anatomy of the rodent scaffold. In our research, we combine the thinned-skull window and the fluid percussion measures to minimize the inflammation due to the craniotomy and the fluid percussion, and produce a more precise model of our repeated mTBI. Overall, the modified closed-head injury associated to repeated mTBI represents a valuable tour for researchers investigating the underlying mechanism that contribute to focal and diffuse pathological changes in repeated mTBI.