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Journal
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Medicine
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AAV Isolation by Double Iodixanol Density Gradient Centrifugation
/
Author Spotlight: Efficient Adeno-Associated Virus Isolation for Pre-Clinical Applications
JoVE Journal
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JoVE Journal
Medicine
Suspension Culture Production and Purification of Adeno-Associated Virus by Iodixanol Density Gradient Centrifugation for In Vivo Applications
Author Spotlight: Efficient Adeno-Associated Virus Isolation for Pre-Clinical Applications
AAV Isolation by Double Iodixanol Density Gradient Centrifugation
DOI:
10.3791/66460-v
•
01:19 min
•
February 09, 2024
•
Kathryn K. Harris
,
Oleksandr Kondratov
,
Sergei Zolotukhin
1
Department of Pediatrics
,
University of Florida
Tags
Adeno-associated Virus
AAV
Gene Therapy
Iodixanol Density Gradient Centrifugation
Recombinant AAV
Suspension Culture
Expi293 Cells
PEG Precipitation
Buffer Exchange
Virus Purification
Virus Production
Virus Yield
In Vivo Applications
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Author Spotlight: Efficient Adeno-Associated Virus Isolation for Pre-Clinical Applications
Double Iodixanol Centrifugation to Purify Adeno-Associated Virus for In Vivo Studies
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