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An Efficient Method for Adenovirus Production

An Efficient Method for Adenovirus Production

Article DOI: 10.3791/61691-v
June 10th, 2021


Summary June 10th, 2021

Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.


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