Name: Techniques for Rapidly Sampling Six Crucial Organs in Adult Xenopus (Video) | JoVE Journal
Uploaded: 2024-02-16T15:00:00.000+00:00
Duration: 5 min 10 s
Description: Learn standardized techniques for rapidly sampling six organs in adult Xenopus, including heart, liver, pancreas, kidneys, fat bodies, and skin. This method enables clean tissue extraction for proteomics and immunochemistry research.

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5 minutes

This work was supported by NIH&#39;s OD grant R24OD031956. We thank Samantha Jalbert, Jill Ralston, and Cora Anderson for their assistance and support as well as our editor and anonymous peer reviewers for their helpful feedback

All experiments were performed in accordance with the rules and regulations of the Harvard Medical School IACUC (Institutional Animal Care and Use Committee) (IS 00001365_3). The representative results are shown for both a perfused and unperfused mature albino male Xenopus laevis.
1. Experimental preparation 
NOTE: If perfusion protocol8 is being followed before sampling, skip to step 2.2.
<ol>
	<li>Ensure that the research institution has approved the euthanasia technique described in this protocol.</li>
	<li>Prepare a solution of 5 g/L MS-222 (tricaine methanesulfonate) and 5 g/L sodium bicarbonate (see Table of Materials). The volume must be greater than the volume required to cover the animals being euthanized completely. Check the pH to ensure that it is &#8805;7.</li>
	<li>Perform primary euthanasia by placing the Xenopus in the euthanasia solution; the animal will remain submerged for a total of 1 h.</li>
	<li>Set up the dissection station so that immediately after sampling, all tissues can be rinsed in chilled PBS or 0.7x PBS9 (depending on experimental needs), checked, and trimmed under a 5x (or greater) magnification light. This station must also enable the user to either replace all forceps and scissors or wipe them clean between uses.</li>
	<li>Once the frog has been in the solution for 1 h, primary euthanasia has been completed. Remove the frog and check the loss of pain response by performing a foot pinch.</li>
	<li>Record the appropriate details for the animal, such as species, strain, sex, age, and health status as well as if it was perfused. Weigh the Xenopus and take any additional measurements, such as snout-vent length.</li>
	<li>Place the frog on its back and pin down the limbs proximal to the body (Figure 1).</li>
	<li>Using dissection scissors, cut through the skin, up the midline, and then laterally, making two flaps.</li>
	<li>Referring to Figure 2, identify the linea alba and use forceps to grasp it and pull it away from the coelomic cavity. Cut up through the musculature carefully using scissors. Make two flaps out of the cavity wall. Cut or pin all flaps out of the way.</li>
	<li>Identify the heart which will still be beating. Use dissecting scissors to reduce the coracoid bones (Figure 2) to gain better access to the heart. 
	NOTE: If the heart has stopped beating before sampling, it should be noted that the sample freshness has been compromised.</li>
</ol>
2. Sampling
NOTE: If the animal has been perfused, skip to step 2.2.
<ol>
	<li>Identify the thin pericardium and pull it taut with tissue forceps (Figure 3).</li>
	<li>Using the tip of the iridectomy scissors, gently perforate the pericardium, being careful not to cut the underlying tissues. Peel the pericardium up away from the 3 chambers of the heart.</li>
	<li>Use forceps to grasp the ventricle by the apex, identify where it attaches to the auricles and arterial trunk (Figure 4), and cut it below these attachments (Figure 5). If required, trim the ventricle so that no tissues from the auricles or arterial trunk are visible, and light-colored valve tissue will still be visible inside the ventricle. 
	NOTE: In unperfused animals, the removal of the ventricle may qualify as secondary euthanasia.</li>
	<li>The 3 lobes of the liver will be visible (Figure 6 and Figure 7). Grasp the lip of the left lobe (on the viewer&#39;s right) and gently lift it so that the hepatic and cystic ducts are visible (Figure 8). Sample the bottom 1/3 of the lobe below these attachments (Figure 9).</li>
	<li>To gain better access to the tissues of a female frog, it is helpful to remove the ovary. Identify the ovary which is enveloped in a layer of visceral peritoneum called the germinal epithelium. Gently shift the lobes until they are on their respective sides to make the area of attachment visible (Figure 10). These attachments are directly ventral to the paired kidney.</li>
	<li>Using scissors, remove the ovaries as close to the kidneys as possible without damaging them (Figure 11).</li>
	<li>Inspect the medial lobe (also called the anterior lobe) of the liver and note how it connects to the stomach and duodenum through the mesentery and hepatopancreatic duct (also called the common bile duct) (Figure 6, Figure 7, and Figure 8).&#160;</li>
	<li>Sever the mesentery, hepatoduodenal ligament using iridectomy scissors as well as the hepatopancreatic duct where it meets the duodenum. Sever the connection of the pancreas and hepatopancreatic duct to the medial lobe of the liver so that no dark liver tissue is attached (Figure 12).</li>
	<li>Grasp the stomach with toothed forceps and the superior end of the pancreas with tissue forceps. Under 5x magnification, gently tease the pancreas off of the stomach (Figure 13). 
	NOTE: If it does not come away cleanly, the remaining pancreatic tissue will be visible and can be picked off in fragments. Alternatively, the pancreas can be methodically detached using iridectomy scissors and tissue forceps.</li>
	<li>Referring to Figure 14A, identify the urinary bladder and remove it, cutting as close to the cloaca as possible. Discard this tissue.</li>
	<li>Referring to Figure 14B, identify the large intestine and pull it taut to sever the large intestine as close to the cloaca as possible. Remove and discard the entire alimentary canal, severing the peritoneum where it attaches to the spleen. The fat bodies will now be fully accessible.</li>
	<li>Tease apart the fat bodies so that they are on their respective sides. The area over the kidney, where the fat body connects to the peritoneum, will be visible. Grasp the base of the left fat body (on the viewer&#39;s right) and use scissors to cut it away from the peritoneum, leaving a small margin so that the kidney is not damaged (Figure 15).</li>
	<li>Remove and discard the remaining fat body. The paired kidneys will now be fully visible.</li>
	<li>In female frogs or males with distinct vestigial oviducts, grasp an oviduct and pull it away from the kidney and cloaca (Figure 16). Cut the oviduct where it meets the cloaca and continue to pull it away from the kidney, cutting any clear peritoneal attachments as they become apparent. Discard this tissue.</li>
	<li>Repeat this process with the remaining oviduct.</li>
	<li>The kidneys are still covered with clear peritoneum (retroperitoneal)10. Use forceps to grasp the kidneys and sever the peritoneum at their inferior end.</li>
	<li>Lift the kidneys out of the coelomic cavity, using scissors to sever the peritoneum as close to the kidneys as possible without damaging them (Figure 17).</li>
	<li>Under 5x magnification, cut away excess peritoneum and any other remaining tissue (fat bodies, spleen). If the frog is female, ensure any remaining ovary tissue is removed (Figure 18). If the frog is male, carefully remove the testis and check for a vestigial oviduct, which may not be visible without magnification (Figure 19).</li>
	<li>Remove the pins from the animal, flip it onto its ventrum, and re-pin the animal&#39;s limbs.</li>
	<li>Select either hindlimb to sample from and pin the foot of that limb.</li>
	<li>Remove an almond-shaped flap of skin from over the gastrocnemius/tibiofibula (Figure 20).</li>
</ol>

Standardized Dissection and Sampling Protocol for Adult Xenopus Organs

<ol><li>Porro, L. B., Richards, C. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Digital+dissection+of+the+model+organism+Xenopus+laevis+using+contrast-enhanced+computed+tomography%5BTitle%5D)+AND+%22J+Anat%22%5BJournal%5D)">Digital dissection of the model organism Xenopus laevis using contrast-enhanced computed tomography.</a> J Anat. 231 (2), 169-191 (2017).</li>
<li>Ruehl-Fehlert, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Revised+guides+for+organ+sampling+and+trimming+in+rats+and+mice--part+1%5BTitle%5D)+AND+%22Exp+Toxicol+Pathol%22%5BJournal%5D)">Revised guides for organ sampling and trimming in rats and mice--part 1.</a> Exp Toxicol Pathol. 55 (23), 91-106 (2003).</li>
<li>Kittel, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Revised+guides+for+organ+sampling+and+trimming+in+rats+and+mice--Part+2.+A+joint+publication+of+the+RITA+and+NACAD+groups%5BTitle%5D)+AND+%22Exp+Toxicol+Pathol%22%5BJournal%5D)">Revised guides for organ sampling and trimming in rats and mice--Part 2. A joint publication of the RITA and NACAD groups.</a> Exp Toxicol Pathol. 55, 413-431 (2004).</li>
<li>Morawietz, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Revised+guides+for+organ+sampling+and+trimming+in+rats+and+mice+-+Part+3+-+A+joint+publication+of+the+RITA+and+NACAD+groups%5BTitle%5D)+AND+%22Exp+Toxicol+Pathol%22%5BJournal%5D)">Revised guides for organ sampling and trimming in rats and mice - Part 3 - A joint publication of the RITA and NACAD groups.</a> Exp Toxicol Pathol. 55, 433-449 (2004).</li>
<li>Patmann, M. D., Shewade, L. H., Schneider, K. A., Buchholz, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Xenopus+tadpole+tissue+harvest%5BTitle%5D)+AND+%22Cold+Spring+Harb+Protoc%22%5BJournal%5D)">Xenopus tadpole tissue harvest.</a> Cold Spring Harb Protoc. 2017 (11), 097675(2017).</li>
<li>Lőw, P., Molnár, K., Kriska, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dissection+of+a+Frog+%28Rana+sp.%29%5BTitle%5D)+AND+%22Atlas+of+Animal+Anatomy+and+Histology%22%5BJournal%5D)">Dissection of a Frog (Rana sp.).</a> Atlas of Animal Anatomy and Histology. , 213-263 (2016).</li>
<li>O'Rourke, D. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Amphibians+used+in+research+and+teaching%5BTitle%5D)+AND+%22ILAR+J%22%5BJournal%5D)">Amphibians used in research and teaching.</a> ILAR J. 48 (3), 183-187 (2007).</li>
<li>Jonas-Closs, R. A., Peshkin, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effective+rapid+blood+perfusion+in+Xenopus%5BTitle%5D)+AND+%22JoVE%22%5BJournal%5D)">Effective rapid blood perfusion in Xenopus.</a> JoVE. (issue), e65287(2023).</li>
<li>Balls, M., Worley, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Amphibian+cells+in+vitro.+II.+Effects+of+variations+in+medium+osmolarity+on+a+permanent+cells+line+isolated+from+Xenopus%5BTitle%5D)+AND+%22Exp+Cell+Res%22%5BJournal%5D)">Amphibian cells in vitro. II. Effects of variations in medium osmolarity on a permanent cells line isolated from Xenopus.</a> Exp Cell Res. 76 (2), 333-336 (1973).</li>
<li>Holz, P. H., Raidal, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparative+renal+anatomy+of+exotic+species%5BTitle%5D)+AND+%22Vet+North+Am+Exot+Anim+Pract%22%5BJournal%5D)">Comparative renal anatomy of exotic species.</a> Vet North Am Exot Anim Pract. 9 (1), 1-11 (2006).</li>
<li>Trott, K. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+a+Mycobacterium+ulcerans-like+infection+in+a+colony+of+African+tropical+clawed+frogs+%28Xenopus+tropicalis%29%5BTitle%5D)+AND+%22Comp+Med%22%5BJournal%5D)">Characterization of a Mycobacterium ulcerans-like infection in a colony of African tropical clawed frogs (Xenopus tropicalis).</a> Comp Med. 54 (3), 309-317 (2004).</li>
<li>Fremont-Rahl, J. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mycobacterium+liflandii+outbreak+in+a+research+colony+of+Xenopus+%28Silurana%29+tropicalis+frogs%5BTitle%5D)+AND+%22Vet+Pathol%22%5BJournal%5D)">Mycobacterium liflandii outbreak in a research colony of Xenopus (Silurana) tropicalis frogs.</a> Vet Pathol. 48 (4), 856-867 (2011).</li>
<li>Green, S. L., Parker, J., Davis, C., Bouley, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ovarian+hyperstimulation+syndrome+in+gonadotropin-treated+laboratory+South+African+clawed+frogs+%28Xenopus+laevis%29%5BTitle%5D)+AND+%22J+Am+Assoc+Lab+Anim+Sci%22%5BJournal%5D)">Ovarian hyperstimulation syndrome in gonadotropin-treated laboratory South African clawed frogs (Xenopus laevis).</a> J Am Assoc Lab Anim Sci. 46 (3), 64-67 (2007).</li>
<li>Vitt, L. J., Caldwell, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Anatomy+of+amphibians+and+reptiles.+Herpetol%5BTitle%5D)+AND+%22Herpetol%22%5BJournal%5D)">Anatomy of amphibians and reptiles. Herpetol.</a> Herpetol. , 35-81 (2009).</li>
</ol>

The authors declare no competing interests.

As this protocol aims to maximize freshness, some samples may include undesired tissues. For example, the hepatopancreatic duct and some mesentery are sampled with the pancreas, and some peritoneal tissue, adrenal glands, and ureters will always be sampled with the paired kidneys.&#160; If freshness is not a concern, then more precise sampling can be achieved using modified techniques.
The appearance and location of organs are comparable between sexes and species of Xenopus. However, the color of tissues varies significantly based on whether or not the animals have been perfused. It is for this reason that images of both perfused and unperfused animals are included.
A constraint of this protocol is that speed and reproducibility are prioritized over collecting samples that best represent the entirety of the desired tissue. For example, the section of the left lobe of the liver sampled here cannot adequately represent all three lobes of liver tissue. If there are errors in sampling, options for troubleshooting are affected by the potential for variation between different sections of tissue. For example, it is not known if the right lobe of the liver, the right fat body, or a different portion of skin would be functional alternatives to the desired tissues. In these cases, discretion should be used, based on the needs of the research, before substituting sections of tissue.
Another limitation of this protocol is that if the animals being sampled have drastic anatomical defects or clinically significant health issues, the organs in the coelomic cavity may not appear as described here. Granulomas have been found in the tissues of frogs infected with Mycobacterium spp.11,12, and previous cases of ovarian hyperstimulation syndrome appear to lead to an abnormal presentation of organs13.
Though this method has been developed for laboratory Xenopus, there are significant similarities in the appearance of these organs within many non-caecilian amphibians and limbed reptiles14. The sampling portion of this protocol could easily be modified for other models, such as axolotls or the green anole.

Though the &#34;digital dissection&#34; of adult Xenopus&#8221; is available1, replicable organ and tissue sampling of adult Xenopus remains challenging without the detailed instruction available for other adult models (e.g. mice2,3,4). This article aims to provide clear guidance for accurate and replicable organ sampling of adult Xenopus similar to what is currently available for their larvae5. An emphasis is placed on ease of completion to maintain maximum freshness and make the protocol accessible to all users.
Though there is a thorough dissection guide for Rana sp.6, as well as numerous classroom dissection guides for other anurans7, no Xenopus dissection and sampling guide is currently available. For those not familiar with sampling practices or amphibian anatomy, the small differences between Xenopus and other anurans render these resources suboptimal for replicable tissue sampling.
Many valuable tissues are not included and are even discarded in the present guide; this is to ensure tissue freshness. Six samples are limited enough to ensure that these tissues can be collected in under an hour after the heart starts beating, regardless of the experience or skill level of the user. More advanced and detailed guides for collecting many other tissues are under preparation as separate companion papers.
For less experienced users, it is always recommended that this protocol be first attempted on animals being euthanized for reasons other than experimentation before sampling any animals that are challenging to replace (i.e., transgenics, animals of advanced age, etc). Ideally, all animals sampled will be healthy and, if female, will not have been ovulated in the past two weeks.

<table><tbody><tr><td>5x Magnifying Glass with LED Light and Stand</td><td>amazon.com</td><td>B08QJ6J8P1</td><td>light must not produce heat</td></tr><tr><td>Disposable Transfer Pipets</td><td>VWR</td><td>414004-036</td><td></td></tr><tr><td>Dissecting Fine-Pointed Forceps</td><td>Fisher Scinetific</td><td>08-875</td><td></td></tr><tr><td>Dissecting scissors sharp piont, straight 6.5&quot;</td><td>VWR</td><td>76457-374</td><td></td></tr><tr><td>Dissection Tray</td><td>Fisher Scinetific</td><td>14-370-284</td><td>styrofoam sheets are an acceptable alternative</td></tr><tr><td>Euthanasia container</td><td>US Plastic&amp;nbsp;</td><td>Item&amp;nbsp;2860</td><td>alternative opaque containers acceptable</td></tr><tr><td>Euthanasia container lid</td><td>US Plastic&amp;nbsp;</td><td>Item&amp;nbsp;3047</td><td></td></tr><tr><td>Iridectomy Scissors 6&quot;</td><td>vwr</td><td>470018-938</td><td>iris scissors are an acceptable alternative</td></tr><tr><td>MS-222: Syncaine (formerly tricaine)</td><td>Pentair AES</td><td>TRS1</td><td></td></tr><tr><td>PBS 1x</td><td>Corning</td><td>21-040-CV</td><td></td></tr><tr><td>Sodium Bicarbonate, Powder, USP</td><td>Fisher Scientific</td><td>18-606-333</td><td></td></tr><tr><td>Specimen Forceps, Serrated</td><td>VWR</td><td>82027-442</td><td></td></tr><tr><td>T-Pins for Dissecting</td><td>Fisher Scinetific</td><td>S99385</td><td></td></tr></tbody></table>

techniques for rapidly sampling six crucial organs in adult xenopus

Techniques for Rapidly Sampling Six Crucial Organs in Adult Xenopus

Xenopus has been a powerful model organism for understanding vertebrate development and disease for over a hundred years. While experimental analysis and dissection techniques of the embryo have been well documented, descriptions of adult Xenopus structures and organs, together with techniques for working with adults, have not been updated to take into consideration the requirements of such modern approaches as quantitative proteomics and single-cell transcriptomics. The cell-type and gene-centric perspectives require contrasting observations in embryonic stages to those in adult tissues. The organs of the larva undergo significant changes in their overall structure, morphology, and anatomical location all along the larval to adult transition, most notably during massive metamorphosis remodeling. Establishing robust standards for organ identification and dissection is crucial to ensure datasets resulting from studies performed at different laboratories can be consistent. The present protocol identifies six of the organs in the adult Xenopus, demonstrating methods for dissection and sampling of the heart ventricle, liver, fat body, pancreas, paired kidney, and skin of the adult Xenopus. Depending on the preservation methods, the dissected organs can be used for quantitative proteomics, single cell/nuclei transcriptomics, in situ hybridization, immunohistochemistry, histology, etc. This protocol aims to standardize tissue sampling and facilitate multi-lab investigations of the adult organ systems.

By utilizing Figure 1 to Figure 20 and following all steps of this protocol, the heart ventricle, the left lobe of the liver, the pancreas, the left fat bodies, paired kidneys, and a flap of skin were cleanly excised within an hour of euthanasia. Within this time, the samples are rinsed and trimmed so that they will appear, as shown in Figure 21.
<img alt="Frog dissection diagram, anatomy study with pinned limbs; measurement lines on abdomen for education." class="xfigimg" src="/files/ftp_upload/66489/66489fig1.JPG" data-altupdatedat="1788840098000" data-alt="Figure 1"> 
Figure 1: Pinned Xenopus. A mature female X. tropicalis pinned through each limb. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig1large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Frog dissection diagram showing coracoid bones, linea alba, for anatomical study and education." class="xfigimg" src="/files/ftp_upload/66489/66489fig2.jpg" data-altupdatedat="1788840098000" data-alt="Figure 2"> 
Figure 2: Abdominal wall. The ventral skin of an X. tropicalis female is cut into flaps, making the linea alba and coracoid bones visible. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Dissection diagram of pericardium and heart in a rat, showing anatomical features and structures." class="xfigimg" src="/files/ftp_upload/66489/66489fig3.JPG" data-altupdatedat="1788840098000" data-alt="Figure 3"> 
Figure 3: Pericardium enclosed heart. The apex of the heart ventricle is grasped through the pericardium. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig3large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Dissection diagram showing arterial trunk and heart ventricle in amphibian anatomy study." class="xfigimg" src="/files/ftp_upload/66489/66489fig4.JPG" data-altupdatedat="1788840098000" data-alt="Figure 4"> 
Figure 4: Heart ventricle and arterial trunk. The ventricle of a perfused X laevis, being grasped, showing its attachment to the arterial trunk. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig4large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Heart anatomy diagram; shows auricles, arterial trunk, ventricle; educational labeling." class="xfigimg" src="/files/ftp_upload/66489/66489fig5.JPG" data-altupdatedat="1788840098000" data-alt="Figure 5"> 
Figure 5: Heart diagram. A diagram of the relevant structures of the heart with a dashed line indicating where to sample the ventricle. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig5large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Liver anatomy diagram with gallbladder, pancreas, ducts, and duodenum, showing bile flow." class="xfigimg" src="/files/ftp_upload/66489/66489fig6.JPG" data-altupdatedat="1788840098000" data-alt="Figure 6"> 
Figure 6: Hepatopancreatic diagram. A diagram of the 3 lobes of the liver, pancreas, and associated organs. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig6large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>

<img alt="Internal organs diagram showing liver, pancreas, spleen, gallbladder, ducts for educational anatomy." class="xfigimg" src="/files/ftp_upload/66489/66489fig7.JPG" data-altupdatedat="1788840098000" data-alt="Figure 7"> 
Figure 7: Hepatopancreatic organs. A perfused, albino X. laevis male with 3 lobes of liver, pancreas, and associated organs labeled. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig7large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Anatomy diagram of frog dissection showing liver lobes, gallbladder, pancreas, and intestines." class="xfigimg" src="/files/ftp_upload/66489/66489fig8.JPG" data-altupdatedat="1788840098000" data-alt="Figure 8"> 
Figure 8: Cystic and hepatic ducts. The left lobe of the liver is being lifted to show the cystic and hepatic ducts in perfused X. laevis. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig8large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Frog dissection showing internal organs, educational biology experiment, anatomy study assistance." class="xfigimg" src="/files/ftp_upload/66489/66489fig9.JPG" data-altupdatedat="1788840098000" data-alt="Figure 9"> 
Figure 9: Liver sampling. The left liver lobe of an unperfused X. tropicalis is severed under the attachments of the hepatic ducts. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig9large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Frog dissection diagram with labeled fat bodies, spleen, germinal epithelium, and ovaries." class="xfigimg" src="/files/ftp_upload/66489/66489fig10.JPG" data-altupdatedat="1788840098000" data-alt="Figure 10"> 
Figure 10: Ovary attachment. With the ovary lobes on their respective sides, the continuity of the germinal epithelium to the peritoneal wall (over the kidneys) is visible. Two white dashed lines indicate where to sever these attachments.  <a href="https://www.jove.com/files/ftp_upload/66489/66489fig10large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Frog anatomy dissection diagram showing labeled organs including liver, stomach, ovaries, intestines." class="xfigimg" src="/files/ftp_upload/66489/66489fig11.JPG" data-altupdatedat="1788840098000" data-alt="Figure 11"> 
Figure 11: Ovary removal. The ovary of an unperfused X. laevis, is pulled away from the paired kidneys. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig11large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Cat dissection anatomy diagram with labeled liver, pancreas, stomach, duodenum; educational resource." class="xfigimg" src="/files/ftp_upload/66489/66489fig12.JPG" data-altupdatedat="1788840098000" data-alt="Figure 12"> 
Figure 12: Mesentery incisions. The coelomic cavity of an unperfused X. laevis, following the sampling of the heart ventricle and left lobe of the liver as well as the removal of the ovary. A white dashed line indicates where to sever the hepatopancreatic ligament and duct, while a green dashed line indicates where to sever the pancreas from the medial lobe of the liver. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig12large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Anatomy dissection showing visceral organs, digestive system components, and connective tissues." class="xfigimg" src="/files/ftp_upload/66489/66489fig13.jpg" data-altupdatedat="1788840098000" data-alt="Figure 13"> 
Figure 13: Pancreas sampling. The pancreas of an unperfused X. laevis is being teased off of the stomach. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig13large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Dissection comparison of urinary bladder and large intestine in pig anatomy diagram." class="xfigimg" src="/files/ftp_upload/66489/66489fig14.JPG" data-altupdatedat="1788840098000" data-alt="Figure 14"> 
Figure 14: Organ removal. (A) The urinary bladder of an unperfused X. laevis is pulled away from the cloaca with a dashed line indicating where to cut it. (B) The large intestine of an unperfused X. laevis, is being pulled away from the cloaca with a dashed line indicating where to sever it. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig14large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Dissection diagram: amphibian internal organs labeled, including testis and kidneys for educational study." class="xfigimg" src="/files/ftp_upload/66489/66489fig15.JPG" data-altupdatedat="1788840098000" data-alt="Figure 15"> 
Figure 15: Fat body sampling. The fat bodies, attached to the peritoneum at the superior end of the paired kidneys, are pulled out of the coelomic cavity with a dashed line showing where to cut them. Note that adjacent to this attachment, this male X. tropicalis has 1 testis as well as a pair of distinct vestigial oviducts. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig15large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Anatomy diagram of reptile dissection; shows right oviduct, clear peritoneum, paired kidneys." class="xfigimg" src="/files/ftp_upload/66489/66489fig16.JPG" data-altupdatedat="1788840098000" data-alt="Figure 16"> 
Figure 16: Oviduct removal. The oviduct of a perfused X. laevis is tugged away from the paired kidney, making the clear peritoneum visible. A dashed line indicates where to incise the peritoneum. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig16large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Dissection diagram showing lungs, paired kidneys, peritoneum, and sciatic plexus anatomy." class="xfigimg" src="/files/ftp_upload/66489/66489fig17.JPG" data-altupdatedat="1788840098000" data-alt="Figure 17"> 
Figure 17: Kidney sampling. The paired kidneys of an unperfused X. laevis are being lifted out of the coelomic cavity. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig17large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Tissue comparison; parasitic infection analysis; biological specimen image; research study result." class="xfigimg" src="/files/ftp_upload/66489/66489fig18.jpg" data-altupdatedat="1788840098000" data-alt="Figure 18"> 
Figure 18: Kidney trimming. (A) A ventral view of an unperfused female X. laevis’s paired kidney with associated peritoneal organs attached. (B) The same kidney with associated organs removed but with some peritoneal tissue remaining. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig18large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Dissection setup, labeled testis and paired kidneys in petri dish, organ identification study." class="xfigimg" src="/files/ftp_upload/66489/66489fig19.JPG" data-altupdatedat="1788840098000" data-alt="Figure 19"> 
Figure 19: Testis removal. The paired kidneys of unperfused X. tropicalis with one testis removed. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig19large.JPG" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Frog leg anatomy comparison; dissected limbs showing muscle structure; biological study." class="xfigimg" src="/files/ftp_upload/66489/66489fig20.jpg" data-altupdatedat="1788840098000" data-alt="Figure 20"> 
Figure 20: Skin sampling. (A) The right leg of an X. tropicalis with a dashed line indicating the area of skin to be sampled. (B) The right leg of an X. tropicalis with a skin sample removed over the tibiofibula. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig20large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Male albino X. laevis organs comparison, perfused vs. not, in petri dishes; biological study." class="xfigimg" src="/files/ftp_upload/66489/66489fig21.jpg" data-altupdatedat="1788840098000" data-alt="Figure 21"> 
Figure 21: Representative results of organ sampling. Samples of heart ventricle, liver, pancreas, fat body, paired kidney, and skin taken from a perfused and unperfused albino X. laevis. <a href="https://www.jove.com/files/ftp_upload/66489/66489fig21large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

This article presents a guide for sampling six significant and diverse organs in adult Xenopus that can be rapidly and easily accessed: the heart ventricle, liver lobe, pancreas, fat bodies, paired kidneys, and skin.

Xenopus has been a powerful model organism for understanding vertebrate development and disease for over a hundred years. While experimental analysis and ...

techniques-for-rapidly-sampling-six-crucial-organs-in-adult-xenopus

Research

JoVE Journal

Biology

This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

Technique to Target Microinjection to the Developing Xenopus Kidney

This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

We describe steps that enable fast in situ sampling of a small portion of an individual cell with high precision and minimal invasion using capillary-based micro-sampling, to facilitate chemical characterization of a snapshot of metabolic activity in live embryos using a custom-built single cell capillary electrophoresis and mass spectrometry platform.

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Presented here is an effective rapid blood perfusion protocol to prepare tissue samples from African clawed frogs for transcriptomics and proteomics studies.

Effective Rapid Blood Perfusion in Xenopus

This is part one of a comprehensive Xenopus sampling protocol. The tissues sampled are the heart ventricle, arterial trunk, left liver lobe, gallbladder, lung, pancreas, spleen, larynx, esophagus, stomach, intestines, testes, fat bodies, oviduct, paired kidneys, sciatic plexus, skin, thymus, and whole eye.

Efficient Techniques for Comprehensive Sampling of Accessible Tissues in Adult Xenopus

Visualization of in vivo RNA transport is accomplished by microinjection of fluorescently labeled RNA transcripts into Xenopus oocytes, followed by confocal microscopy.

Visualizing RNA Localization in Xenopus Oocytes

This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.

Dissection of Organs from the Adult Zebrafish

Stimulus-evoked [Ca2+]i signals of individual human sperm are assessed. Motile cells are loaded with Ca2+-sensitive fluorescent dye (AM-ester method) and immobilised in a perfusable chamber. Cells are imaged by time-lapse fluorescence microscopy and stimulated via the perfusing medium. Responses of single cells (or regions) are analysed offline using Excel.

Techniques for Imaging Ca2+ Signaling in Human Sperm

Ionic current of BK channels is recorded using patch clamp techniques. BK channels are expressed in Xenopus oocytes by injecting messenger RNA. The intracellular solution during patch clamp recordings is controlled by a perfusion system.

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

Learn standardized techniques for rapidly sampling six organs in adult Xenopus, including heart, liver, pancreas, kidneys, fat bodies, and skin. This method enables clean tissue extraction for proteomics and immunochemistry research.

Techniques for Rapidly Sampling Six Crucial Organs in Adult Xenopus

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