Xenopus Dissection

Xenopus dissection is the systematic examination of a Xenopus frog’s external and internal anatomy, providing a practical way to connect biological structure with function. During the procedure, the specimen is humanely immobilized or preserved according to approved protocols, then opened with careful incisions so students or researchers can expose and identify organs, tissues, and body systems while minimizing damage to key structures. In biology education, this preparation supports comparative anatomy, developmental biology, and studies of vertebrate organization, while helping learners develop observation, anatomical orientation, and scientific documentation skills. Because Xenopus shares many fundamental vertebrate features, dissection also provides context for interpreting morphology and experimental research findings.

Xenopus Dissection - Related Videos

Research

JoVE Journal - Neuroscience

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

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Cited by 6 •

2012

Xenopus laevis provides an ideal model system for studying cell fate specification and physiological function of individual retinal cells in primary cell culture. Here we present a technique for dissecting retinal tissues and generating primary cell cultures that are imaged for calcium activity and analyzed by in situ hybridization.

Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

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Cited by 2 •

2007

This video demonstrates the technique used for preparation of organizer and animal pole explants from Xenopus laevis embryos, including the use of the eyebrow knife - a specialized dissection tool made of one's eyebrow. The protocol for assembling an adhesion assay is also given, which probes for the presence of key adhesion molecules present on the surface organizer or animal pole cells that are critical for proper development.

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

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Cited by 22 •

2014

This protocol describes how to dissect premigratory cranial neural crest (NC) from Xenopus laevis neurulas. These explants can be plated on fibronectin and cultured in vitro, or grafted back into host embryos. This technique allows studying the mechanisms of NC epithelium-to-mesenchyme transition, migration, and differentiation.

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

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Cited by 2 •

2017

This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

Functional Cloning Using a Xenopus Oocyte Expression System

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Cited by 3 •

2016

We describe a Xenopus oocyte and animal cap system for the expression cloning of genes capable of inducing a response in competent ectoderm, and discuss techniques for the subsequent analysis of such genes. This system is useful in the functional identification of a wide range of gene products.

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