Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection

Lei Xu; Manman Xu; Yinghai Xie; Wei You; Jianxin Wang; Liwu Xu; Qizhu Feng; Jie Sun; Jian Zhang; Huaichen Yang; Wang Qi

doi:10.3791/66780

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Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection

595 Views

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07:38 min

•

May 10, 2024

DOI:

10.3791/66780-v

DOI:

10.3791/66780-v

•

07:38 min

May 10, 2024

•

536 Views

Lei Xu*^1,2, Manman Xu*³, Yinghai Xie⁴, Wei You², Jianxin Wang², Liwu Xu⁴, Qizhu Feng⁴, Jie Sun⁴, Jian Zhang⁴, Huaichen Yang⁴, Wang Qi⁴

¹The First Affiliated Hospital of Anhui University of Science & Technology ²Medical College of Anhui University of Science & Technology ³Endocrinology Department,The First Affiliated Hospital of Anhui University of Science & Technology ⁴Department of Hepatobiliary Surgery,The First Affiliated Hospital of Anhui University of Science & Technology

Summary

Intraperitoneal drug administration is a safe and effective non-invasive approach for inducing pancreatic injury. This study compared five distinct intraperitoneal injection protocols on mice to induce varying degrees of pancreatic injury and established a model of severe pancreatic injury to investigate the pathological changes and treatment strategies for severe acute pancreatitis (SAP).