511 Views
•
09:01 min
•
January 24, 2025
DOI:
Summary
Automatically generated
Here, we present a protocol to investigate the interaction of primary human monocyte-derived macrophages with a tumor spheroid in a three-dimensional (3D) collagen I matrix, with the possibility to compare the impact of soluble and physical properties of the microenvironment on cell invasion.
Related Videos
11:24
Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates
Related Videos
6985 Views
10:51
Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
Related Videos
12297 Views
06:11
Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
Related Videos
9757 Views
08:26
A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay
Related Videos
7980 Views
08:49
A 3D Organotypic Melanoma Spheroid Skin Model
Related Videos
15696 Views
08:19
A Spheroid Killing Assay by CAR T Cells
Related Videos
16250 Views
09:57
Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
Related Videos
22462 Views
08:15
Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
Related Videos
15514 Views
10:33
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
Related Videos
27398 Views
06:27
A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
Related Videos
8839 Views
Read Article
Cite this Article
Hey, S., Linder, S. A Macrophage-Tumor Spheroid Co-Invasion Assay. J. Vis. Exp. (215), e67374, doi:10.3791/67374 (2025).
Copy