Laser Scanning Microscopy
Laser Scanning Microscopy - Related Videos
09:44
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
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Cited by 20 •
2011
We present a protocol for structural and compositional analysis of natural oral biofilm from orthodontic appliances with in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). Oral biofilm samples were collected from palatal expanders, scraping acrylic-resin flakes off their surface and referring them for molecular processing.
09:41
Using Laser Scanning Microscopy to Determine Electromigration in Molybdenum Disilicide
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2025
Here, we describe a workflow using laser scanning microscopy to determine the volume electromigrated through a metal line under test. By varying different experimental variables, a multitude of information about electromigration can be acquired. In this work, the length of the onset of electromigration is determined.
09:20
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
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Cited by 11 •
2020
Presented here is the protocol of the Conpokal technique, which combines confocal and atomic force microscopy into a single instrument platform. Conpokal provides same cell, same region, near simultaneous confocal imaging and mechanical characterization of live biological samples.
11:41
Scanning Electron Microscopy (SEM)
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2023
Source: Laboratory of Dr. Andrew J. Steckl — University of Cincinnati A scanning electron microscope, or SEM, is a powerful microscope that uses electrons to form an image. It allows for imaging of conductive samples at magnifications that cannot be achieved using traditional microscopes. Modern light microscopes can achieve a magnification of ~1,000X, while typical SEM can reach magnifications of more than 30,000X. Because the SEM doesn’t use light to create images, the resulting pictures it...
09:50
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
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Cited by 6 •
2011
This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.
FAQs
During scanning, a focused laser beam interrogates one location at a time rather than illuminating the entire specimen simultaneously. Detectors record the light emitted or reflected from each position, allowing the system to build an image from spatially organized measurements. This approach helps reveal fine biological organization and preserves information about where signals occur within cells or tissues.
Optical filters help select the relevant fluorescent signal from the light collected by the microscope. In confocal systems, a pinhole suppresses light originating outside the focal region, reducing out-of-focus contributions. The resulting optical sections appear sharper because signals from different depths interfere less with one another, improving visualization of structures within three-dimensional biological specimens.
The microscope collects optical sections at different positions through a specimen. These separate images can then be combined to reconstruct the specimen in three dimensions. Such reconstructions allow researchers to examine the spatial relationships among cells, tissues, or labeled molecules, rather than relying only on a single two-dimensional view of biological organization.
Detection depends on the type of signal produced by the specimen. Fluorescence applications measure emitted light from labeled molecules, with optical filtering used to isolate the relevant signal. Other measurements can use reflected light from the specimen itself. These alternatives allow laser scanning microscopy to examine biological structures through either molecular labeling or reflected optical information.
A typical workflow places the specimen in the microscope, focuses the laser on the region of interest, and scans the sample point by point. Detectors collect emitted or reflected light, while filtering and, when applicable, a confocal pinhole help refine the signal. Researchers can then examine individual optical sections or combine them into a three-dimensional reconstruction.
The method supports cell biology, neuroscience, developmental biology, and pathology. In these fields, researchers can investigate how structures are arranged, how labeled molecules change, and how biological processes unfold across space. Three-dimensional imaging is especially useful when relationships among cells or tissues matter, while repeated observations can help examine dynamic processes and disease-associated changes.