Name: Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy (Video) | JoVE Journal
Uploaded: 2025-02-28T12:40:00.000+00:00
Duration: 5 min 6 s
Description: Learn non-invasive capillaroscopy to visualize nailbed microvascular morphology in mice. This method enables easy assessment of peripheral vascular dysfunction for neurodegenerative disease research.

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5 minutes

This work was funded by unrestricted departmental funds awarded to Lauren K. Wareham. Dr. Pasquale is supported by The Glaucoma Foundation (NYC) and by an unrestricted challenge grant from Research to Prevent Blindness (NYC).

All methods described here have been approved by the Institutional Animal Care and Use Committee (IACUC) of Vanderbilt University Medical Center and Massachusetts General Hospital.
1. Preparation of mouse nails for imaging
NOTE: For optimal vessel clarity and skin recovery, allow at least 24 h before imaging.
<ol>
	<li>To enable unobstructed imaging of mouse nailbeds, remove fur from the rodent paws at least 24 h before capillaroscopy imaging (Figure 1A). To remove fur from mouse paws, follow steps 1.1.1-1.1.6.
	<ol>
		<li>Sedate the animal using inhaled isoflurane anesthesia (2% isoflurane in 5% carbon dioxide/95% oxygen). Confirm that the mouse is adequately anesthetized by pinching the foot pad on both hind feet. If properly anesthetized, there should be no jerking motion (pedal withdrawal reflex.) If there is a reflex movement, allow the mouse more time under isoflurane to become fully anesthetized. Re-test for the pedal withdrawal reflex and continue.</li>
		<li>After the mouse is properly anesthetized, apply a lubricant eye gel or sterile non-medicated ophthalmic ointment to both eyes to prevent drying of the cornea while under anesthesia.</li>
		<li>Maintaining the animal under anesthesia using a nose cone, apply a generous amount of hair removal cream to the entire paw using an applicator. Take care to cover the entire paw and nailbed areas, as shown in Figure 1B.</li>
		<li>Leave hair removal cream on the paw for 1 min at room temperature (RT). If hair can be easily removed at this time, then remove hair and rinse with copious amounts of water.</li>
		<li>Carefully clean away hair removal cream by gently rubbing it with a clean tissue.</li>
		<li>Wash the paw in lukewarm, sterile water. 
		NOTE: Paws should be free of fur and nails unobstructed for imaging, as shown in Figure 1C.</li>
		<li>Return the mouse to its cage to ensure a safe recovery from anesthesia. Do not leave the mouse unattended until it has regained consciousness. Once the mouse has regained consciousness, return it to the original cage with the company of other animals.</li>
	</ol>
	</li>
</ol>
2. In vivo nailbed capillaroscopy imaging
<ol>
	<li>Following a minimum of 24 h recovery time post-fur removal, set up the capillaroscopy equipment in a temperature-controlled room (maintained between 21.5-22.5 &#176;C), as shown in Figure 2A.The complete setup for nailbed imaging includes 1) isoflurane anesthesia equipment, 2) an anesthesia nose cone, 3) an adjustable animal stage, 4) a capillaroscopy microscope, and 5) a laptop with video software for imaging.</li>
	<li>Sedate the animal using inhaled isoflurane anesthesia (2% isoflurane in 5% carbon dioxide/95% oxygen).
	<ol>
		<li>Confirm that the mouse is adequately anesthetized by pinching the foot pad on both hind feet. If properly anesthetized, there should be no jerking motion (pedal withdrawal reflex.) If there is a reflex movement, return to step 1.1.1 and allow the mouse more time to be fully anesthetized. Then, re-test for the pedal withdrawal reflex and continue.</li>
		<li>After the mouse is properly anesthetized, apply lubricant eye gel or sterile non-medicated ophthalmic ointment to both eyes to prevent drying of the cornea during anesthesia.</li>
	</ol>
	</li>
	<li>Maintaining the animal under sedation, position the hind paw volar side up on top of the lab tape platform below the objective, as shown in Figure 2B, zoom.</li>
	<li>Spread the toes gently to separate nails under the microscope objective using an applicator. Ensure nailbeds are separated from one another for optimal vessel imaging. 
	NOTE: Figure 2C shows the complete imaging setup showing vessel picture on laptop video software.</li>
	<li>To reduce glare and improve focus, generously apply immersion oil (corn oil) to the paw, ensuring complete nail coverage. Add white tape or similar under the mouse paw to enhance contrast and improve visualization of the vessel bed (Figure 3; arrow 3).</li>
	<li>Focus on the nail on the second digit of the hind paw; in mice, this is the largest nail and easiest to image. To focus the mouse nail, use the x and y stage adjustors (Figure 3; arrow 4) and magnification wheel (up to 280x on this instrument; Figure 3; arrow 1).</li>
	<li>Turn the objective to reduce the positioning of glare to bring the nail vessel network into view (Figure 3; arrow 2). 
	NOTE: If vessels become difficult to see or imaging time becomes extended, generously reapply the immersion oil. Font paws of mice are smaller than the hind paws; thus, it is recommended that imaging is carried out on hind paws.</li>
	<li>Connect the capillaroscope to a laptop computer via a universal serial bus (USB) connection.</li>
	<li>Open the Debut video software application on the laptop. 
	NOTE: Verify that the device is properly connected to the laptop in settings.</li>
	<li>On the laptop screen, visualize what is being magnified by the capillaroscope and focus on the nailbed by adjusting the x and y stage adjustors and the magnification wheel to obtain a clear image.</li>
	<li>Once the microscope is focused and a clear image of the vessel network in the nailbed is seen, record a video by hitting the red record button in Debut or a similar video software program (Figure 2C).</li>
	<li>Save each video to the appropriate project folder and label each video accordingly.</li>
</ol>
3. Saving nailbed images
<ol>
	<li>Open the nailbed video in the software and manually choose a frame in the video where vessels are in clear focus.</li>
	<li>Using the Screenshot tool on the computer, take a screenshot of the debut video screen showing clear vasculature in the nail. Save image.</li>
	<li>Open the screenshot image in ImageJ software by clicking File and Open; select the file from its destination folder.</li>
	<li>If required, adjust the brightness and contrast by selecting Image &#62; Adjust &#62; Brightness/Contrast. This tool can help alter the contrast of the images to better visualize the vessel morphology.</li>
	<li>Once the image has been adjusted, click Set in the Brightness/Contrast tool.</li>
	<li>Save the image as a TIFF file by clicking on File and then Save as.</li>
</ol>

<ol><li>El Miedany, Y., Ismail, S., Wadie, M., Hassan, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nailfold+capillaroscopy%3A+tips+and+challenges%5BTitle%5D)+AND+%22Clin+Rheumatol%22%5BJournal%5D)">Nailfold capillaroscopy: tips and challenges.</a> Clin Rheumatol. 41 (12), 3629-3640 (2022).</li>
<li>Etehad Tavakol, M., Fatemi, A., Karbalaie, A., Emrani, Z., Erlandsson, B. -E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nailfold+capillaroscopy+in+rheumatic+diseases%3A+Which+parameters+should+be+evaluated%5BTitle%5D)+AND+%22BioMed+Res+Int%22%5BJournal%5D)">Nailfold capillaroscopy in rheumatic diseases: Which parameters should be evaluated.</a> BioMed Res Int. 2015 (1), 974530(2015).</li>
<li>Cutolo, M., Smith, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Detection+of+microvascular+changes+in+systemic+sclerosis+and+other+rheumatic+diseases%5BTitle%5D)+AND+%22Nat+Rev+Rheumatol%22%5BJournal%5D)">Detection of microvascular changes in systemic sclerosis and other rheumatic diseases.</a> Nat Rev Rheumatol. 17 (11), 665-677 (2021).</li>
<li>Lim, M. W. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nailfold+video-capillaroscopy+in+the+study+of+cardiovascular+disease%3A+a+systematic+review%5BTitle%5D)+AND+%22Blood+Press+Monit%22%5BJournal%5D)">Nailfold video-capillaroscopy in the study of cardiovascular disease: a systematic review.</a> Blood Press Monit. 28 (1), 24-32 (2023).</li>
<li>Philip, S., Najafi, A., Tantraworasin, A., Pasquale, L. R., Ritch, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nailfold+capillaroscopy+of+resting+peripheral+blood+flow+in+exfoliation+glaucoma+and+primary+open-angle+glaucoma%5BTitle%5D)+AND+%22JAMA+Ophthalmol%22%5BJournal%5D)">Nailfold capillaroscopy of resting peripheral blood flow in exfoliation glaucoma and primary open-angle glaucoma.</a> JAMA Ophthalmol. 137 (6), 618-625 (2019).</li>
<li>Taniguchi, E. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Peripheral+microvascular+abnormalities+associated+with+open-angle+glaucoma%5BTitle%5D)+AND+%22Ophthalmol+Glaucoma%22%5BJournal%5D)">Peripheral microvascular abnormalities associated with open-angle glaucoma.</a> Ophthalmol Glaucoma. 6 (3), 291-299 (2023).</li>
<li>Maldonado, G., Guerrero, R., Paredes, C., Ríos, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nailfold+capillaroscopy+in+diabetes+mellitus%5BTitle%5D)+AND+%22Microvasc+Res%22%5BJournal%5D)">Nailfold capillaroscopy in diabetes mellitus.</a> Microvasc Res. 112, 41-46 (2017).</li>
<li>Gurfinkel, Y. I., Sasonko, M., Priezzhev, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Digital+capillaroscopy+as+important+tool+for+early+diagnostics+of+arterial+hypertension+Proc%5BTitle%5D)+AND+%22SPIE+9448%2C+Saratov+Fall+Meeting+2014%3A+Optical+Technologies+in+Biophysics+and+Medicine+XVI%3B+Laser+Physics+and+Photonics+XVI%3B+and+Computational+Biophysics%22%5BJournal%5D)">Digital capillaroscopy as important tool for early diagnostics of arterial hypertension Proc.</a> SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics. , 944804(2015).</li>
<li>Santamaría, R., González-Álvarez, M., Delgado, R., Esteban, S., Arroyo, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Remodeling+of+the+microvasculature%3A+May+the+blood+flow+be+with+you%5BTitle%5D)+AND+%22Front+Physiol%22%5BJournal%5D)">Remodeling of the microvasculature: May the blood flow be with you.</a> Front Physiol. 11, 586852(2020).</li>
<li>Pries, A. R., Secomb, T. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Making+microvascular+networks+work%3A+angiogenesis%2C+remodeling%2C+and+pruning%5BTitle%5D)+AND+%22Physiology%22%5BJournal%5D)">Making microvascular networks work: angiogenesis, remodeling, and pruning.</a> Physiology. 29 (6), 446-455 (2014).</li>
<li>Cutolo, M., Smith, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((State+of+the+art+on+nailfold+capillaroscopy%3A+a+reliable+diagnostic+tool+and+putative+biomarker+in+rheumatology%5BTitle%5D)+AND+%22Rheumatology%22%5BJournal%5D)">State of the art on nailfold capillaroscopy: a reliable diagnostic tool and putative biomarker in rheumatology.</a> Rheumatology. 52 (11), 1933-1940 (2013).</li>
<li>Bentley, M. D., Ortiz, M. C., Ritman, E. L., Romero, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+use+of+microcomputed+tomography+to+study+microvasculature+in+small+rodents%5BTitle%5D)+AND+%22Am+J+Physiol+Regul+Integr+Comp+Physiol%22%5BJournal%5D)">The use of microcomputed tomography to study microvasculature in small rodents.</a> Am J Physiol Regul Integr Comp Physiol. 282 (5), R1267-R1279 (2002).</li>
<li>Zagorchev, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Micro+computed+tomography+for+vascular+exploration%5BTitle%5D)+AND+%22J+Angiogenes+Res%22%5BJournal%5D)">Micro computed tomography for vascular exploration.</a> J Angiogenes Res. 2, 7(2010).</li>
<li>Untracht, G. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pilot+study+of+optical+coherence+tomography+angiography-derived+microvascular+metrics+in+hands+and+feet+of+healthy+and+diabetic+people%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Pilot study of optical coherence tomography angiography-derived microvascular metrics in hands and feet of healthy and diabetic people.</a> Sci Rep. 13 (1), 1122(2023).</li>
<li>Mirg, S., Turner, K. L., Chen, H., Drew, P. J., Kothapalli, S. -R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Photoacoustic+imaging+for+microcirculation%5BTitle%5D)+AND+%22Microcirculation%22%5BJournal%5D)">Photoacoustic imaging for microcirculation.</a> Microcirculation. 29 (6-7), e12776(2022).</li>
<li>Gkontra, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dynamic+changes+in+microvascular+flow+conductivity+and+perfusion+after+myocardial+infarction+shown+by+image-based+modeling%5BTitle%5D)+AND+%22J+Am+Heart+Assoc%22%5BJournal%5D)">Dynamic changes in microvascular flow conductivity and perfusion after myocardial infarction shown by image-based modeling.</a> J Am Heart Assoc. 8 (7), e011058(2019).</li>
<li>Laurent, S., Agabiti-Rosei, C., Bruno, R. M., Rizzoni, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microcirculation+and+macrocirculation+in+hypertension%3A+A+dangerous+cross-link%5BTitle%5D)+AND+%22Hypertension%22%5BJournal%5D)">Microcirculation and macrocirculation in hypertension: A dangerous cross-link.</a> Hypertension. 79 (3), 479-490 (2022).</li>
<li>Kisler, K., Nelson, A. R., Montagne, A., Zlokovic, B. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cerebral+blood+flow+regulation+and+neurovascular+dysfunction+in+Alzheimer+disease%5BTitle%5D)+AND+%22Nat+Rev+Neurosci%22%5BJournal%5D)">Cerebral blood flow regulation and neurovascular dysfunction in Alzheimer disease.</a> Nat Rev Neurosci. 18 (7), 419-434 (2017).</li>
<li>Wareham, L. K., Calkins, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+neurovascular+unit+in+glaucomatous+neurodegeneration%5BTitle%5D)+AND+%22Front+Cell+Dev+Biol%22%5BJournal%5D)">The neurovascular unit in glaucomatous neurodegeneration.</a> Front Cell Dev Biol. 8, 452(2020).</li>
<li>Mandujano, A., Golubov, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Animal+models+of+systemic+sclerosis%3A+using+nailfold+capillaroscopy+as+a+potential+tool+to+evaluate+microcirculation+and+microangiopathy%3A+a+narrative+review%5BTitle%5D)+AND+%22Life%22%5BJournal%5D)">Animal models of systemic sclerosis: using nailfold capillaroscopy as a potential tool to evaluate microcirculation and microangiopathy: a narrative review.</a> Life. 12 (5), 703(2022).</li>
<li>Kim, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nail+fold+capillaroscopy+as+a+potential+tool+to+evaluate+breast+tumor%5BTitle%5D)+AND+%22J+Anal+Sci+Technol%22%5BJournal%5D)">Nail fold capillaroscopy as a potential tool to evaluate breast tumor.</a> J Anal Sci Technol. 15 (1), 35(2024).</li>
<li>McKay, G. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Visualization+of+blood+cell+contrast+in+nailfold+capillaries+with+high-speed+reverse+lens+mobile+phone+microscopy%5BTitle%5D)+AND+%22Biomed+Opt+Express%22%5BJournal%5D)">Visualization of blood cell contrast in nailfold capillaries with high-speed reverse lens mobile phone microscopy.</a> Biomed Opt Express. 11 (4), 2268-2276 (2020).</li>
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</ol>

Unrelated to this work, Dr. Pasquale was a paid consultant to Twenty Twenty.&#160;Unrelated to this work, Clara Cousins is a paid consultant to Cartography Biosciences. The other authors have nothing to disclose.

In summary, we provide a protocol allowing researchers to easily and inexpensively assess the morphology of mouse nailbed microvasculature, a novel location for non-invasive peripheral vascular imaging. Like NFC methods used in guinea pigs20 and mice21, the major strength of the protocol described here is that it allows for quick and non-invasive evaluation of peripheral microvasculature in mouse models of disease. This could be particularly useful for studies that involve ...

Imaging peripheral microcapillary networks in humans using nailfold capillaroscopy (NFC) has highlighted the importance of microcirculation as a target organ system in a wide range of systemic illnesses1. Capillaroscopy involves the use of a microscope to magnify and visualize vessels in the nailfold in vivo. As such, it is a technique widely used in the clinic to detect peripheral microvascular dysfunction and abnormalities in a range of systemic conditions, including rheumatic2,3, cardiac4, ocular (e.g., glaucoma)5,6, and endocrine diseases (e.g., hypertension and diabetes mellitus7,8). Morphological changes in the nailfold capillaries, including hemorrhages, increased vessel tortuosity, and avascular regions, are readily detected using NFC. These morphological abnormalities represent pathological processes such as excessive or deficient microvascular remodeling9,10. NFC is a useful diagnostic tool for detecting these pathologies. Additionally, this technique is useful in the assessment of drug efficacy11.
However, translating clinical NFC findings to animal models of disease is challenging for many reasons. Visualizing microvasculature in animals is typically invasive (e.g., endoscopic), carried out ex vivo (e.g., post-mortem imaging of tissues), or expensive, requiring specialized equipment such as microcomputed tomography12,13, coherence tomography angiography14, and photoacoustic imaging techniques15. Since peripheral microvascular pathology is evident in a broad range of systemic and central nervous system diseases, including myocardial infarction16, hypertension17, age-related neurodegenerations of the central nervous system such as Alzheimer&#39;s disease18, and optic neuropathies such as glaucoma19, a non-invasive, cost-effective in vivo visualization technique is highly beneficial.
Capillaroscopy has been used to evaluate the nailfold microvasculature in animal models, including guinea pigs20 and mice21, thus demonstrating its capability as a non-invasive imaging tool. Here, we apply capillaroscopy to a different part of the nail, the nailbed. Harnessing the transparency of the mouse nail, nailbed capillaroscopy introduces a novel location for the visualization of peripheral microvasculature. In comparison to NFC, which is particularly useful for monitoring blood cell motion21,22, the nailbed capillaroscopy protocol described here provides a larger area for better observation of microvascular morphology and structure. We provide a protocol that allows researchers to easily and inexpensively assess the morphology of mouse nailbed microvasculature, which is a novel location for non-invasive peripheral vascular imaging. This protocol provides representative images of typical nailbed microvascular architecture in wild-type mice using two commonly used laboratory strains (SV129/S6 and C57/B6J). We show that nailbed capillaroscopy is an inexpensive, non-invasive microvascular imaging modality. Further studies using this exploratory method will be essential to apply nailbed capillaroscopy to a wide range of mouse models of disease where peripheral microvascular abnormalities are evident in pathology.

<table><tbody><tr><td>Anesthetic Charcoal Filter Cannister</td><td>ReFresh</td><td>EZ-258</td><td></td></tr><tr><td>Capillaroscope&amp;nbsp;</td><td>Jiahua Electronic Instrument Co., Jiangsu, China</td><td>JH-1004</td><td></td></tr><tr><td>Compressed gas (5% carbon dioxide, 95% oxygen)</td><td>Airgas</td><td>UN3156</td><td></td></tr><tr><td>Corn oil</td><td>Sigma</td><td>C8-267&amp;nbsp;</td><td></td></tr><tr><td>Debut video capture software</td><td>Debut</td><td></td><td>Available free online.</td></tr><tr><td>Eye spears</td><td>BVI Weck- Cel</td><td>0008680</td><td>For application and removal of hair removal cream.</td></tr><tr><td>Hair removal cream</td><td>Nair&amp;nbsp;</td><td>610370323649</td><td></td></tr><tr><td>Isoflurane 250 mL bottle</td><td>Piramal critical care</td><td>NDC &amp;nbsp;6679401725</td><td></td></tr><tr><td>Lab jack&amp;nbsp;</td><td>Fisherbrand</td><td>14-673-52</td><td>Used as a platform to hold the mouse.</td></tr><tr><td>Nose cone (low profile anesthesia mask)</td><td>Kent Scientific</td><td>SOMNO-0801</td><td></td></tr><tr><td>Transfer pipettes</td><td>Fisherbrand</td><td>13-711-9AM</td><td>Apply corn oil generously to mouse paw as an immersion oil.</td></tr><tr><td>USB Video capture card</td><td>VIXLW</td><td>BR116</td><td></td></tr><tr><td>Vetequip</td><td>VWR</td><td>89012-492</td><td>Isoflurane equipment</td></tr><tr><td>White labeling tape&amp;nbsp;</td><td>Fisherbrand</td><td>15-958</td><td>Used to create a white/contrasting background under mouse paw when taking images.</td></tr></tbody></table>

non-invasive visualization of nailbed microvascular morphology in mice using capillaroscopy

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy

Imaging microcapillary networks of the skin in humans using nailfold capillaroscopy (NFC) has underscored the importance of microcirculation as a target organ system in critical systemic illnesses. Nailfold capillaroscopy is applied clinically to detect peripheral microvascular dysfunction and abnormalities in a range of systemic conditions, including rheumatic, cardiac, ocular (e.g., glaucoma), and endocrine disorders (e.g., hypertension and diabetes mellitus). NFC is useful not only in detecting peripheral systemic microvasculature disruption but also in assessing drug efficacy. However, translating clinical NFC findings to animal disease models can be challenging. Detecting microvascular dysfunction or abnormalities in animals is often invasive (e.g., endoscopic), carried out ex vivo (e.g., post-mortem imaging of tissues), or expensive, requiring specialized equipment such as those used in microcomputed tomography and photoacoustic imaging techniques. Developing quick, non-invasive, and inexpensive techniques to image peripheral microvasculature in animal models of disease is warranted to decrease research expenses and increase translatability to the clinic.
Capillaroscopy has previously been used to visualize the nailfold microvasculature in animal models, including in guinea pigs and mice, thus demonstrating the capability of capillaroscopy as a non-invasive imaging tool in animal models. This study provides a protocol that applies capillaroscopy to a mouse nailbed, allowing researchers to easily and inexpensively assess the morphology of its microvasculature. Representative images of typical nailbed microvascular architecture in wild-type mice using two commonly used laboratory strains, SV129/S6 and C57/B6J, are provided. Further studies using this method are essential for applying nailbed capillaroscopy to a wide range of mouse disease models with peripheral microvascular abnormalities.

Using the capillaroscopy method described here, nailbed vascular morphology can be easily imaged, as shown in Figure 4A. Typical nailbed vasculature in a mouse exhibits three consistent features, as highlighted in Figure 4B: each nailbed has 1) an afferent vessel, 2) an efferent vessel, and 3) a network of capillaries connecting both the afferent and efferent vessels. To demonstrate the consistency of nailbed morphology, we show in Figure 4C...

Capillaroscopy is an accessible tool for direct, inexpensive, and non-invasive visualization of microvasculature. The goal of this protocol is to enable researchers to use capillaroscopy for the visualization of peripheral microvascular morphology in the nailbeds of mice.

Imaging microcapillary networks of the skin in humans using nailfold capillaroscopy (NFC) has underscored the importance of microcirculation as a target ...

non-invasive-visualization-nailbed-microvascular-morphology-mice

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