Trypsinizing and Subculturing Mammalian Cells

Journal

Biology

JoVE Journal
Biology

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JoVE Journal Biology

Trypsinizing and Subculturing Mammalian Cells

DOI:

10.3791/755-v

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05:59 min

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June 12, 2008

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Richard Ricardo, Katy Phelan

¹Molecular Pathology Laboratory Network, Inc

Chapters

00:00Title
00:37Introduction
00:56Trypsinizig and Subculturing Mammalian cells from a Monolayer
04:00Passaging Cells in Suspension Culture
05:36Conclusion

Summary

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As cells reach confluency, they must be subcultured or passaged. This video will demonstrate a procedure for subculturing both adherent and suspension cells.

Trypsinizing and Subculturing Mammalian Cells

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