Freezing, Thawing, and Packaging Cells for Transport

Richard Ricardo; Katy Phelan

doi:10.3791/757

Freezing, Thawing, and Packaging Cells for Transport

JoVE Journal
Biology

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JoVE Journal Biology

Freezing, Thawing, and Packaging Cells for Transport

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07:32 min

July 2, 2008

DOI: 10.3791/757-v

Richard Ricardo¹, Katy Phelan¹

¹Molecular Pathology Laboratory Network, Inc

Cultured mammalian cells are used extensively in cell biology studies. This video describes the basic skills required to freeze and store cells and how to recover frozen stocks.

Cell cultures are utilized In a wide variety of research and diagnostic applications. It is sometimes desirable to store cell lines for future work to preserve cells, avoid senescence, reduce the risk of contamination, and minimize the effects of genetic drift. Cell lines may be frozen for long-term storage.

In this video, we show you how to freeze cells grown both in monolayer and in suspension, and how to thaw them for growth once again. Hi, I'm Ricky here at the Dr.LAN's laboratory at Molecular Pathology Laboratories Network in East Tennessee. Today I'm going to show you how to freeze cells for long-term storage and how to thaw them from liquid nitrogen.

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