Oct4 Ssea4
Oct4 Ssea4 - Related Videos
09:45
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
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Cited by 17 •
2017
This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.
09:57
Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
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Cited by 16 •
2016
We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.
09:56
Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
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2017
Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.
08:58
Induced Pluripotency
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2023
Induced pluripotent stem cells (iPSCs) are somatic cells that have been genetically reprogrammed to form undifferentiated stem cells. Like embryonic stem cells, iPSCs can be grown in culture conditions that promote differentiation into different cell types. Thus, iPSCs may provide a potentially unlimited source of any human cell type, which is a major breakthrough in the field of regenerative medicine. However, more research into the derivation and differentiation of iPSCs is still needed to...
08:56
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
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Cited by 5 •
2016
The protocol presented in this study describes methods for the real-time monitoring of reprogramming progression via the kinetic measurement of positive and negative pluripotent stem cell markers using flow cytometry analysis. The protocol also includes the imaging-based assessment of morphology, and marker or reporter expression during iPSC generation.
FAQs
Using both markers provides complementary evidence when researchers assess a stem cell culture. Oct4 reflects regulation of gene expression linked with self-renewal and pluripotency, while SSEA-4 supplies a cell-surface readout detected with antibodies. Their combined assessment can strengthen evaluation of stem cell identity and support quality control in embryonic and induced pluripotent stem cell cultures.
Changes in the two marker signals can help researchers follow the transition from a pluripotent state during directed differentiation. Because Oct4 is associated with self-renewal and pluripotency and SSEA-4 is detected at the cell surface, monitoring both provides complementary information about changing cell characteristics rather than relying on a single readout.
SSEA-4 adds a surface-associated measurement to an assessment that includes Oct4-related gene regulation. Researchers can detect it through antibody-based immunostaining or flow cytometry, allowing the marker to contribute an independent perspective on cell identity. This complementarity is useful when evaluating cultures, reprogramming progress, or changes associated with differentiation.
During reprogramming, researchers examine whether marker patterns change in a direction consistent with acquisition of stem cell characteristics. Oct4 contributes information about gene expression associated with self-renewal and pluripotency, while SSEA-4 provides a cell-surface signal. Together, these measurements help evaluate reprogramming progress and support decisions about culture quality.
Antibody-based immunostaining and flow cytometry are established approaches for examining the marker set described here. SSEA-4 can be detected through either method, while Oct4 is assessed as part of the combined marker analysis. Selecting between these approaches allows researchers to organize marker evaluation around their culture-monitoring or identity-assessment workflow.
Assessment of these markers supports quality control in embryonic and induced pluripotent stem cell cultures and helps track changes during directed differentiation. The same strategy contributes to studies of early development, disease modeling, and regenerative biology. In each context, the markers help researchers evaluate stem cell identity or follow changes relevant to the experimental system.