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From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

Article DOI: 10.3791/832-v • 03:51 min • June 10th, 2008
June 10th, 2008 •
1, 1, 1
1David Geffen School of Medicine, University of California, Los Angeles

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This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. Part 3 of 3.

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MEFs Matrigel HESCs Feeder Cell-free Conditions Passage Growth Pluripotency Immunofluorescence Microscopy
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