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21:24 min
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January 02, 2009
DOI:
1Department of Cancer Genetics and Developmental Biology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Department of Pathology and Laboratory Medicine,University of British Columbia – UBC, 4Photography/Video Production, Multi-Media Services,BC Cancer Agency, 5Department of Medical Genetics, Life Sciences Institute,,University of British Columbia – UBC
Summary
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This video demonstrates the protocol for methylated DNA immunoprecipitation (MeDIP). MeDIP is a two day procedure that selectively extracts methylated DNA fragments from a genomic DNA sample using antibodies with specificity for 5 -methylcytosine (anti-5 mC).
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Methylated DNA ImmunoprecipitationDNA Methylation PatternsEpigeneticsGene ExpressionNormal DevelopmentDiseaseMethylation ProfilesMeDIPMethylated DNA ExtractionAntibody ReactionImmunoprecipitated DNAInput DNAHeat DenaturationAnti-5’mC AntibodyMagnetic BeadsSecondary AntibodyAntibody ComplexSeparation Of Methylated DNANon-methylated DNA Removal
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Cite this Article
Thu, K. L., Vucic, E. A., Kennett, J. Y., Heryet, C., Brown, C. J., Lam, W. L., Wilson, I. M. Methylated DNA Immunoprecipitation. J. Vis. Exp. (23), e935, doi:10.3791/935 (2009).
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