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Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)

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Biology

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JoVE Journal Biology

Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)

Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)

DOI:

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11:49 min

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January 30, 2009

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Neela Zareen, Lloyd A. Greene

¹Department of Biology,Columbia University, ²Department of Pathology and Cell Biology,Columbia University

Chapters

00:05Title
00:53Introduction
01:30Dissection of Superior Cervical Ganglia
05:33Cleaning the Ganglia
06:23Establishing Sympathetic Neuron Culture
10:06Conclusion

Summary

Automatic Translation

This is a protocol describing how to isolate and culture primary sympathetic neurons from superior cervical ganglia (SCG) of newborn rat pups.

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