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Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)
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Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)

Article DOI: 10.3791/988 • 11:49 min • January 30th, 2009
January 30th, 2009 •
1, 2
1Department of Biology, Columbia University, 2Department of Pathology and Cell Biology, Columbia University

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Summary
Automatic Translation

This is a protocol describing how to isolate and culture primary sympathetic neurons from superior cervical ganglia (SCG) of newborn rat pups.

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Culturing Sympathetic Neurons Rat Superior Cervical Ganglia SCG Nerve Growth Factor NGF Culture Experimental Manipulation Neuronal Development Differentiation Programmed Cell Death Pathological Cell Death Signal Transduction Dissecting Newborn Rats Pups Establish Cultures Preparatory Steps Reagents Equipment
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