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Quantifizierung und Analyse der Bildung von HO-Endonuklease Stimulated chromosomalen Translokationen von Single-Strand Annealing in Saccharomyces cerevisiae
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Quantifizierung und Analyse der Bildung von HO-Endonuklease Stimulated chromosomalen Translokationen von Single-Strand Annealing in Saccharomyces cerevisiae

Article DOI: 10.3791/3150-v • 09:41 min • September 23rd, 2011
September 23rd, 2011 •
1,2, 2, 3, 2
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology, City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology, University of Southern California, Norris Comprehensive Cancer Center

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Die HO-stimulierte Translokation Assay überwacht Einzelstrang-Glühen nach der Gründung von DNA-Doppelstrangbrüche an mehreren Loci in diploiden

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Genetik Translokation Bildung HO-Endonuklease Genomic Southern-Blot Chromosome Blot Pulsed-Field-Gelelektrophorese homologe Rekombination DNA-Doppelstrangbrüche einsträngige RNA-Glühen
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