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JoVE Journal Neuroscience
Patch-clamp Kapasitans Målinger og Ca 2 + Imaging ved Singel nerveterminaler i Retinal Slices
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Patch-clamp Kapasitans Målinger og Ca 2 + Imaging ved Singel nerveterminaler i Retinal Slices

Article DOI: 10.3791/3345-v • 09:17 min • January 19th, 2012
January 19th, 2012 •
1, 1, 1
1The Vollum Institute, Oregon Health and Science University

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Her beskriver vi en protokoll for utarbeidelse av agar-embedded retinal skiver som er egnet for elektrofysiologi og Ca2 + bildebehandling. Denne metoden gjør det mulig å studere bånd-type synapser i retinal microcircuits bruke direkte patch-clamp opptak av single presynaptiske nerveterminaler.

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Nevrovitenskap synaptic fysiologi axon terminal synaptic bånd retina microcircuits gullfisk sebrafisk hjerne skiver patch-clamp membran kapasitans målinger kalsium-imaging eksocytose sender utgivelsen
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