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JoVE Journal
Immunology and Infection

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Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
 

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Article DOI: 10.3791/4097-v 13:45 min July 23rd, 2012
July 23rd, 2012

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Summary

Microglia are resident macrophages that provide the first line of defense and immune surveillance of the central nervous system. MicroRNAs are regulatory molecules that play an important role in many physiological processes including activation and differentiation of macrophages. In this article, we describe the method for measurement of microRNAs in microglia.

Tags

MicroRNAs Microglia Real-time PCR Neuroinflammation CNS CD11b CD45 Activation Markers Pathological Events Phenotype Functions MiRNAs Cell Growth Differentiation Inflammation Innate Immune Cells Macrophages MRNA Expression Analysis
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