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Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
JoVE Journal
Biology
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JoVE Journal Biology
Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
DOI:

14:02 min

November 01, 2012

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Chapters

  • 00:05Title
  • 02:22Preparing Intact Muscle Bundles
  • 05:55Measuring Contractility of the Isolated Skeletal Muscles
  • 11:25Results: Ex Vivo Assessment of Contractility, Fatigability and Alternans in Murine Skeletal Muscles
  • 12:19Conclusion

Summary

Automatic Translation

We describe a method to directly measure muscle force, muscle power, contractile kinetics and fatigability of isolated skeletal muscles in an in vitro system using field stimulation. Valuable information on Ca2+ handling properties and contractile machinery of the muscle can be obtained using different stimulating protocols.

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