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JoVE Journal Neuroscience
Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain
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Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

Article DOI: 10.3791/52853-v • 06:58 min • June 1st, 2015
June 1st, 2015 •
1, 1, 2, 3, 1
1Department of Pharmacology and Experimental Therapeutics, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Life Sciences Institute, University of Michigan, 3Department of Biomedical & Pharmaceutical Sciences, Chapman University, School of Pharmacy, Rinker Health Science campus

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Summary
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Using high-resolution differential interference contrast (DIC) microscopy, an ex vivo observation of the beating of motile ependymal cilia located within the mouse brain ventricles is demonstrated by live-imaging. The technique allows a recording of the unique ciliary beating frequency and beating angle as well as their intracellular calcium oscillation pacing properties.

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Live Imaging Ependymal Cilia Lateral Ventricles Mouse Brain Neurological Deficits Ex Vivo Motile Ependymal Cilia Technique Mice Euthanasia Carbon Dioxide Craniectomy Brain Removal Sagittal Brain Dissection Vibratome Sharp Blade Thin Sections Brain Lateral Ventricles Visualization Of Cilia Incubation Glass-bottom Plate Dulbecco's Modified Eagle's Medium (DMEM) High-resolution Differential Interference Contrast Microscope Ciliary Beating Frequency Classification Of Ependymal Cells High-speed Fluorescence Imaging Analysis
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